| 流式细胞术检测细胞凋亡比较研究 |
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| 引用本文： | 王珊,李宁,于力方,廖杰.流式细胞术检测细胞凋亡比较研究[J].标记免疫分析与临床,2005,12(3):168-170. |
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| 作者姓名： | 王珊 李宁 于力方 廖杰 |
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| 作者单位： | 解放军总医院医学实验测试中心,北京,100853;解放军总医院医学实验测试中心,北京,100853;解放军总医院医学实验测试中心,北京,100853;解放军总医院医学实验测试中心,北京,100853 |
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| 摘 要： | 从三方面探讨检测细胞凋亡流式细胞术的方法.选用大鼠皮质神经元细胞,同时使用碘化丙锭(PI)An-nexin V/PI.JC-1法检测细胞凋亡率.结果显示三种方法的凋亡率分别是:2.60%,6.52%和18.56%,两两之间有显著性差异(P＜0.01).JC-1法最敏感,AV/PI次之,PI单染法不适合检测早期细胞凋亡.
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| 关 键 词： | 流式细胞术 细胞凋亡 碘化丙锭 Annexin V JC-I |
| 文章编号： | 1006-1703(2005)03-0168-03 |
| 收稿时间： | 2005-02-16 |
| 修稿时间： | 2005年2月16日 |
Study on Comparison of Methods for Detection of Cell Apoptosis by Flow Cytometry |
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| WANG Shan, LI Ning, YU Li-fang,et al..Study on Comparison of Methods for Detection of Cell Apoptosis by Flow Cytometry[J].Labeled Immunoassays and Clinical Medicine,2005,12(3):168-170. |
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| Authors: | WANG Shan LI Ning YU Li-fang |
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| Institution: | WANG Shan, LI Ning, YU Li-fang, et al. |
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| Abstract: | To evaluate the method of measuring apoptosis by flow cytometry with three stains, the apoptosis percentage of rat cortical neurons was measured by flow cytometry using propidium iodide(PI) staining, Annexin V/PI and JC-1 double staining respectively. The results showed that: the apoptotic positive percentage measured by PI staining,Annexin V/PI and JC-1 was 2.60%,6.52% and 18.56%, respectively. Our conclusion is that there were significant differences among the results of three staining methods: JC-1 staining was the most sensitive method, whereas PI single staining was not suitable to measure the early apoptosis of cells. |
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| Keywords: | Annexin V JC-I |
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