庆大霉素对小鼠螺旋神经节神经元电生理特性的影响

目的 探讨庆大霉素对小鼠耳蜗螺旋神经节神经元细胞电生理特性的影响及其意义。方法 应用全细胞电压钳技术研究庆大霉素对急性酶分离小鼠螺旋神经节神经元细胞膜上的钾、钠离子通道电流的峰值的影响．与细胞外液中庆大霉素浓度的关系，以及庆大霉素洗脱后电流的恢复情况。结果 庆大霉素能抑制电压依赖性钾通道，但不能抑制电压依赖性钠通道，其钾通道抑制作用与细胞外液中庆大霉素的浓度呈剂量依赖性，庆大霉素洗脱后电流恢复不完全。结论 庆大霉素通过抑制螺旋神经节神经元细胞的钾离子通道而产生耳毒性。

Influence of Gentamycin Electrophysiological Properties on Murine Cochlea Spiral Ganglion Neurons

Objective To explore the influence of gentamycin on murine cochlea spiral ganglion neurons' electrophysiological properties and its significance.Methods Using whole-cell voltage clamp technique, we studied gentamycin's influence on the peak currents of the potassium and sodium ion channels on cell membranes of acutely dissociated murine spiral ganglion neurons,the relationship to gentamycin's concentration in extracellular fluid, and the currents' recovery after gentamycin being washed out.Results Gentamycin could inhibit voltage-dependent potassium channels, but it couldn't inhibit voltage-dependent sodium channels. Gentamycin's inhibitation on potassium currents had dose-dependence with gentamycin's concerntration in extracellular fluid and the currents recoverd incompletely after gentamycin being washed out.Conclusion This research explained the ototoxic mechanism of gentamycin through its action on keeping from spiral ganglion neurons' potassium ion channels from the electrophysiological aspect, and set a foundation for further research.