E-选择素基因G98T多态性与原发性高血压发生率的相关性研究

目的:研究年龄在E-选择素G98T基因多态性对高血压影响中的作用。方法:高血压组326例,对照组216例。对2组进行血压测量,检测生化指标及一氧化氮(NO)、内皮素(ET)、血管紧张素Ⅰ(AⅠ)、血管紧张素Ⅱ(AⅡ)、醛固酮(ALD)。以聚合酶链反应-限制片段长度多态性(PCR-RFLP)方法进行E-选择素DNA多态性分析,电泳判断基因型并测序。结果:检测到TT、GG、GT3种基因型。在<40岁组,携带T等位基因发生高血压的危险性为G的6.294倍P=0.007;比值比(OR)=6.294;95%可信区间(CI):1.361~29.11]。在40~60岁及>60岁组,高血压组与对照组的等位基因频率差异无显著性(P=0.528和0.965)。<40岁的HT组中TT-TG基因型诊室收缩压(CSBP)、诊室舒张压(CDBP)及平均动脉压(MAP)显著高于GG型(P分别为0.006、0.023和0.036)。在40~60岁组:TT-TG基因型的CSBP、MAP也显著高于GG基因型(P分别为0.031和0.042),而>60岁组,两基因型间血压差异无显著性。结论:E-选择素G98T基因多态性与高血压显著相关,T等位基因携带者发生高血压风险大,血压值较高。

Correlation between polymorphism of E-selectin G98T and blood pressure in patient with essential hypertension

Objective： To investigate correlation between of E-selectin G98T polymorphism and blood pressure. Method： Genotypes of 326 essential hypertension patients male 173, female 153, age （47.12 ± 13.22） y, BMI （26.16± 3.33）kg/m^2] and 216 control individuals male 60,female 156, age （40.04 ± 11.38）y, BMI （24.82 ± 3.04）kg/m^2] were divided into three age groups, 〈 40 y,40-60 y,and 〉 60 y. The polymorphism were typed by polymerase chain reaction restriction fragment length polymorphism （ PCR- RFLP）. The blood pressure and BG, TG, TC, LDL, HDL, UA, BUN, NO, ET, AI, All, ALD of each individuals were measured. Result： Three genotypes （ TY, GG,GT） were found. In 〈 40 y group, Risk of hypertension for allele T is 6.294 times of that for allele G（ P = 0.007 ;OR = 6.294;95 % CI：1.361-29.11 ）. In 40-60 y and 〉 60 y groups, no significantly difference in genotype were found either in HT patient or control. In HT group aged 〈 40 y, the CSBP,CDBP and MAP in TY-TG genotype are significantly higher than that in GG（ P = 0.006,0.023 and 0. 036）. Conclnsion： E-selectin G98T polymorphism is related with blood pressure. T allele has higher risk for hypertension and higer blood pressure. But this effect are con＇elated with age.