| 黄芪多糖对哮喘小鼠特异性免疫治疗的增强作用 |
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| 引用本文： | 宋泽庆,林璘,朱艳芬. 黄芪多糖对哮喘小鼠特异性免疫治疗的增强作用[J]. 中国免疫学杂志, 2010, 26(2) |
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| 作者姓名： | 宋泽庆 林璘 朱艳芬 |
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| 作者单位： | 广东医学院附属医院呼吸内科,湛江,524000 |
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| 基金项目： | 广东省中医药管理局课题 |
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| 摘 要： | 目的:探讨黄芪多糖(Astragalus Polysaccharide,APS)对哮喘小鼠特异性免疫治疗(Specific Immune Therapy,SIT)的影响及其可能的作用机制。方法:90只4～6周龄SPF级BALB/c小鼠,随机平均分为空白对照组、哮喘模型组、SIT治疗组、APS治疗组、APS和SIT单纯联合治疗、APS和OVA混合液治疗组等6组;用OVA致敏,分别用SIT、APS及两者联合等方法治疗,用1%OVA雾化吸入激发;最后一次激发24小时后处死小鼠,进行BALF细胞计数;HE染色观察肺组织切片炎症细胞浸润程度;ELISA法检测血清IFN-γ、IL-4。结果:SIT、APS及两者联合治疗组肺组织炎症细胞浸润程度减轻,BALF中白细胞总数及嗜酸性粒细胞数减少,血清IFN-γ水平上升,IL-4水平下降,联合治疗组比分别治疗组明显。结论:APS可增强SIT的效果,其机制可能是通过影响IFN-γ、IL-4分泌水平实现。
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| 关 键 词： | 支气管哮喘 特异性免疫治疗 黄芪多糖 |
The enhancement of Astragalus Polysaccharide on specific immunotherapy in asthmatic mouse |
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| SONG Ze-Qing,LIN Lin,ZHU Yan-Fen. The enhancement of Astragalus Polysaccharide on specific immunotherapy in asthmatic mouse[J]. Chinese Journal of Immunology, 2010, 26(2) |
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| Authors: | SONG Ze-Qing LIN Lin ZHU Yan-Fen |
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| Affiliation: | SONG Ze-Qing,LIN Lin,ZHU Yan-Fen.Department of Respiratory Medicine,Affiliated Hospital of Guangdong Medicine College,Zhanjiang 524000,China |
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| Abstract: | Objective:To explore the effects the of Astragalus Polysaccharide on specific immune therapy in a mouse model of asthma.Methods:Ninety SPF(Specific pathogen Free) BALB/c mice were randomly divided into six groups.The experimental groups were sensitized with OVA,treated with SIT or APS or their joint application.Then the mice were excited with 1% OVA.24 hours after the last excition,the numbers of the total inflammation cells and esoinophils(EOS) in BALF were counted.The left lung tissues were used to perfor... |
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| Keywords: | Th1/Th2 Asthma SIT APS Th1/Th2 |
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