不同柴胡组分对大鼠肝毒性与氧化损伤机制影响的研究

目的：比较柴胡不同组分对大鼠肝毒性损伤程度和氧化损伤机制的影响。方法：给大鼠灌胃柴胡醇提和水提组分，柴胡醇提和水提组分按等生药量计算，高、中、低剂量组分别为10.0，5.0，2.5 g·kg^-1，30 d后观察一般状况,检测肝功相关指标、血中总巯基(-SH)、血和肝组织内丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH-Px)的含量和活性。结果：醇提和水提柴胡组分均可导致血中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性增高，肝脏质量增加、肝体比值增大，血中总-SH含量降低，血和肝组织内MDA含量增加，GSH含量降低，SOD和GSH-Px的活性下降；上述变化随剂量增加而逐渐加重，与蒸馏水对照组相比有明显差异。结论：柴胡不同组分均可导致大鼠肝毒性损伤，其途径与过氧化损伤机制有关；且醇提组分的肝毒性损伤程度高于水提组分。

Comparative reseach of different Bupleurum chinense composition to influence of hepatotoxicity of rats and oxidative damage mechanism

Objective: To compare the influence of different Bupleurun chinense composition to the degree of hepatotoxicity damage to rats and oxidative damage mechanism. Method: To successively lavage alcohol extracted and water extracted Bupleurun chinense composition to rats for 30-day, to observe the general condition and detect the related index of liver function, the content of total-SH in serum, the content of MDA, the activity of SOD, the content and activity of GSH and GSH-Px in serum and liver tissue. Result: Alcohol and water extracted Bupleurun chinense composition all can induce the activity of ALT and AST in serum increasing, liver weight and the ratio of liver to body increasing, the content of total -SH in serum decreasing, the content of MDA increasing, the content of GSH decreasing, and the activity of SOD and GSH-Px decreasing in serum and liver tissue; the above-mentioned change gradually aggravates with dose increasing, and it is the obvious discrepancy compared with control group with distilled water. Conclusion: The different Bupleurum chinense composition all can induce hepatotoxicity damage, and the channel of hepatic damage is related with the peroxidative damage mechanism, the degree of hepatotoxicity damage caused by the alcohol extracted composition is super to the water extracted composition.