2型糖尿病患者rs4987318基因多态性和生化指标分析

目的观察L-选择素（L-sellectin,SELL）基因rs4987318单核苷酸多态性（single nucleotide polymor-phism,SNP）与2型糖尿病（type 2 diabetes mellitus,T2DM）的相关性和生化指标的变化。方法应用荧光标记单碱基延伸及寡核苷酸微阵列芯片杂交技术检测SELL基因rs4987318多态性;采用全自动生化分析仪检测血脂及C-反应蛋白（C-reactive protein,CRP）水平。结果 T2DM组CC基因型频率明显高于健康对照组（P〈0.05）。T2DM组的甘油三酯（triglyceride,TG）、载脂蛋白B（apolipoprotein B,Apo B）和CRP水平显著高于对照组（P〈0.05）,高密度脂蛋白胆固醇（high-density lipoprotein cholesterol,HDL-C）和载脂蛋白A1（apolipoprotein A1,Apo A1）水平显著低于对照组（P〈0.05）。T2DM组TG的AA、CA、CC型,Apo B的CC型和CRP的CA、CC型的水平显著高于对照组的相同基因型（P〈0.05）;HDL-C的AA、CA、CC型和Apo A1的AA、CA型水平显著低于对照组的相同基因型（P〈0.05）。结论 SELL基因rs4987318多态性可能与T2DM的易患性、患者血脂代谢紊乱和血管炎症的发生有关。

Rs4987318 Polymorphism and Clinical Biochemical Values in Patients with Type 2 Diabetes Mellitus

Objective To investigate the association between rs4987318 single nucleotide polymorphism(SNP) in L-sellectin(SELL) gene and type 2 diabetes mellitus(T2DM) and the clinical biochemical indexes.Methods The rs4987318 mutations in the SELL gene were analyzed with allele-specific fluorogenic oligonucleotide probes in an assay combining extension and hybridization.The levels of lipid and C-reactive protein(CRP) were detected by automatic biochemical modular system.Results The frequencies of CC genotype of T2DM group was significantly higher than that of the control group(P<0.05).In T2DM group,the serum levels of triglyceride(TG),apolipoprotein B(Apo B) and CRP were higher,and the high-density lipoprotein cholesterol(HDL-C) and apolipoprotein A1(Apo A1) were lower than that in the control group(P<0.05).TG levels of AA,CA and CC genotype,Apo B level of CC genotype,CRP levels of CA and CC genotypes were significantly higher in T2DM group than that of the same genotypes in the control group(P<0.05).HDL-C levels of AA,CA and CC genotypes,Apo A1 levels of AA and CA were significantly lower in T2DM group than that of the same genotypes in the control group(P<0.05).Conclusion Rs4987318 polymorphism in SELL may be associated with T2DM susceptibility,lipid metabolism disorder and vessel inflammation of T2DM patients.