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| HPLC法测定芩榆烧伤液中黄芩苷的含量 | |
| 引用本文： | 李学强,李维强,贺金华.HPLC法测定芩榆烧伤液中黄芩苷的含量[J].解放军药学学报,2012,28(3):250-251. |
| 作者姓名： | 李学强 李维强 贺金华 |
| 作者单位： | 1. 新疆军区联勤部药品仪器检验所, 新疆乌鲁木齐,830063 2. 842000 新疆库车,解放军69220部队医院 3. 新疆维吾尔自治区药物研究所, 新疆乌鲁木齐,830001 |
| 摘 要： | 目的建立芩榆烧伤液中黄芩苷的含量测定方法,方法采用HPLC法,色谱柱:ODS-2 C18(250 mm×4.6 mm,5μm),流动相:甲醇-0.2%磷酸水溶液(45∶55);流速为1.0 ml/min;检测波长为280 nm。结果黄芩苷在8.05～80.50μg/ml范围内呈良好线性关系,r=0.9995;回收率为100.46%,RSD为1.44%。结论该方法灵敏、准确、简单、快速,可用于芩榆烧伤液中黄芩苷的含量测定。 |
| 关 键 词： | 芩榆烧伤液 黄芩苷 HPLC |
Determination of Baicalin in Qinyu Burns Liquid by HPLC |
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| LI Xue-qiang , LI Wei-qiang , HE Jin-hua.Determination of Baicalin in Qinyu Burns Liquid by HPLC[J].Pharmaceutical Journal of Chinese People's Liberation Army,2012,28(3):250-251. | |
| Authors: | LI Xue-qiang LI Wei-qiang HE Jin-hua |
| Institution: | ③ ①Institute for Drug and Intrument Control of Xinjiang Military Command,Urumqi 830063,China; ②Hospital of Army 69220 of PLA,Kuche 842000,China; ③Institute of Pharmacology,Xinjiang Uygur Autonomous Region,Urumqi 830001,China |
| Abstract: | Objective To establish an HPLC method for determination of baicalin in Qinyu Burns Liquid.Methods Hypersil ODS-2 C18(250 mm×4.6 mm,5 μm)was used with the mobile phase consisting of methanol-0.2%H3PO4 solution(45 ∶ 55) at a flow rate of 1.0 ml/min.The detection wavelength was set at 280 nm.Results Baicalin showed good linear relationship in the range of 8.05-80.50 μg/ml,r=0.9995.The average recovery was 100.46% and RSD was 1.44%.Conclusion This method is sensitive,accurate,simple and can be used for baicalin determination. |
| Keywords: | Qinyu Burns Liquid baicalin HPLC |
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