黄蜀葵花黄酮成分F-6对四氯化碳致小鼠急性肝损伤的保护作用

目的观察黄蜀葵花黄酮成分F-6对四氯化碳致小鼠急性肝损伤的保护作用。方法实验分为6组:空白对照组、四氯化碳模型组、联苯双酯阳性对照组、F-6低、中、高剂量组。连续给药7 d后,用四氯化碳制备小鼠急性肝损伤模型。测定各组小鼠血清中谷丙转氨酶、谷草转氨酶活性,测定肝组织匀浆中丙二醛含量、超氧化物歧化酶活性;HE染色观察肝组织病理形态学的改变。结果 F-6能显著降低四氯化碳所致肝损伤小鼠血清中谷丙转氨酶、谷草转氨酶活性及肝组织中丙二醛含量,提高超氧化物歧化酶活性;F-6能减轻四氯化碳引起小鼠肝、脾肿大程度;F-6治疗组小鼠肝组织受损程度显著减轻。结论 F-6对四氯化碳所致的小鼠急性肝损伤具有保护作用,其保肝降酶作用显著,其机制可能与清除自由基、抑制脂质过氧化反应有关。

Protective Effect of F-6 from Abelmoschus Manihot on Acute Liver Injury of Mice Induced by CCl4

Objective To research the protective effect of F-6 from Abelmoschus Manihot on acute liver injury of mice induced by carbon tetrachloride.Methods Mice were divided randomly into six groups:normal group,carbon tetrachloride model group,high,medium and low dose of F-6 groups,and the biphenyldimethylesterate group.An acute liver injury model induced by carbon tetrachloride in mice was established after seven days of medication.Samples of the blood,liver,spleen were collected to study the liver function index(ALT,AST),the content of MDA in the liver,and the activity of SOD.Results F-6 decreased the activity of ALT and AST,reduced the content of MDA and increased the activity of SOD obviously.The pathological observation of the liver showed that F-6 could relieve liver injury obviously.Conclusion F-6 can protect the liver from injury by carbon tetrachloride.The mechanism of F-6 may be related to scavenging free radicals and inhibiting lipid peroxidation.