经皮纤维环穿刺法建立兔椎间盘退行性变动物模型

背景：合适的椎间盘退变动物模型是椎间盘组织工程研究的必要条件，但目前尚缺乏公认的模型制备方法。 目的：采用C形臂辅助下经皮纤维环穿刺法制备兔椎间盘退变动物模型，并评估其可行性。 方法：选定新西兰大白兔L2/3和L3/4椎间盘作为穿刺干预椎间盘，L1/2和L5/6椎间盘作为空白对照组，采用C形臂辅助下经皮穿刺法干预椎间盘。于术后2，4，8，12周各选择2只兔麻醉后拍摄兔腰椎核磁共振影像，处死动物采集椎间盘，进行椎间盘髓核蛋白多糖测定。核磁共振检查观察椎间盘退行性改变，二甲基亚甲蓝染色分光光度法测定髓核中蛋白多糖含量变化。 结果与结论：术后4周穿刺干预椎间盘髓核区域核磁共振信号强度及髓核内蛋白多糖含量同空白对照组相比均下降(P < 0.05)，其后两者呈现逐渐下降趋势。结果证实，C形臂X射线机辅助下经皮纤维环穿刺法可用于椎间盘退变动物模型的制备。

Establishment of rabbit intervertebral disc degeneration models using percutaneous annulus fibrosus puncture

BACKGROUND: Although an advisable experimental animal model is necessary for the intervertebral disc tissue engineering, currently, there is not an accepted experimental animal models preparation method. OBJECTIVE: To detect the feasibility of the establishment of the rabbit intervertebral disc degeneration animal model by means of C-arm-assisted percutaneous annulus fibrosus puncture. METHODS: L2/3 and L3/4 disc of New Zealand white rabbit were selected as puncture intervention discs, the L1/2, L5/6 disc were selected as blank control, the intervention group discs were treated by percutaneous annulus fibrosus puncture with the aids of C-arm. Two rabbits were anesthetized at 2, 4, 8, and 12 weeks respectively after operation for MRI images. Then the animals were killed, the change of lumbar intervertebral discs was observed by MRI, and nucleus proteoglycan contents were measured by dimethylmethyleneblue (DMMB) dye spectrophotometry. RESULTS AND CONCLUSION: Signal intensity and proteoglycan content of the nucleus pulposus of the intervention group showed a progressive downtrend at 4 weeks after intervention (P < 0.05), but, by contrast, signal intensity and proteoglycan content of the nucleus pulposus in the control group were gradually decreased. C-arm-assisted percutaneous annulus fibrosus puncture is a feasible method in intervertebral disc degeneration animal model preparation.