| 树突状细胞抗原负载方法探讨 |
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| 引用本文： | 郭文菁 杨美香 祁澜 曲迅 孔北华. 树突状细胞抗原负载方法探讨[J]. 临床检验杂志, 2007, 25(1): 26-27 |
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| 作者姓名： | 郭文菁 杨美香 祁澜 曲迅 孔北华 |
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| 作者单位： | 山东大学第二医院妇产科,济南,250012;山东大学齐鲁医院临床基础研究所,济南,250012;山东大学齐鲁医院临床基础研究所,济南,250012 |
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| 基金项目： | 国家自然科学基金面上项目（30472261及30671902）. |
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| 摘 要： | 目的 探讨树突状细胞（DC）抗原负载方法。方法 体外诱导培养外周血单核细胞来源的DC，分别用脂质体和Nucleofector转染试剂盆体外负载抗原，比较2种负载方法转染效率以及对DC表型的影响。结果 Nucleofector转染方法能够显著的提高转染效率，2种转染方法对DC细胞的表型无显著影响。结论 Nucleofector转染是一种有效的DC抗原体外负载方法，为抗原修饰的DC细胞疫苗的改进提供了新的途径。
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| 关 键 词： | 树突状细胞 抗原负载 脂质体 Nucleofector |
| 文章编号： | 1001-764X(2007)01-0026-02 |
| 修稿时间： | 2006-03-05 |
Research on methods of antigen transfection into dendritic cells |
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| GUO Wenjing,YANG Meixiang,QI Lan,QU Xun,KONG Beihua. Research on methods of antigen transfection into dendritic cells[J]. Chinese Journal of Clinical Laboratory Science, 2007, 25(1): 26-27 |
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| Authors: | GUO Wenjing YANG Meixiang QI Lan QU Xun KONG Beihua |
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| Affiliation: | 1. Department of Obstetrics and Gynecology,The Second Hospital of Shandong University, Jinan 250012 ;2 Basic Research Institute of Clinic Medicine, Qilu Hospital, Jinan 250012, China |
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| Abstract: | Objective To research the effective method of antigen transfection into dendritic cells(DC).Methods Human dendritic cells were derived from peripheral blood monocytes.The plasmid pEGFP-N1 was transfected into DC with liposome or by Nucleofector Kit.The transfecting efficiency was detected by microscopy and FCM.Results The efficiency of Nucleofector Kit was significantly higher than that of liposome.No significant differences of the cellular morphology and surface molecules expression level of DC were shown between the transfections by liposome and Nucleofector Kit.Conclusion Nucleofector Kit is an effective method for antigen transfection and may provide a new strategy of tansfection for antigen-modified DC vaccine. |
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| Keywords: | dendritic cell antigen transfection liposome Nucleofector Kit |
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