肝癌相关基因HCCA2的克隆及其与肝癌的相关性研究

目的　研究和克隆新的肝癌相关基因，探索肝癌发生的分子基础。方法　采用mRNA差异显示技术，分析肝细胞癌和癌旁组织间基因的差异表达情况，获得差异表达基因片断。用此基因片断作为探针，通过筛选胎盘cDNA文库，以获得该基因cDNA全长，并用Northern印迹杂交的方法，分析所获得的新基因在43对肝细胞癌、对应癌旁组织中的差异表达和在正常组织中的分布。结果　克隆了一个新的肝癌相关基因HCCA2的全长cDNA，其在人体正常组织中分布较为广泛，在正常肝组织中不表达。该基因在43对肝癌组织中表达的阳性率是79%(34/43)。它的表达与肝癌包膜的完整性、Ki-67蛋白的表达相关（P<0.01）。结论　肝癌差异表达新基因HCCA2可能与肝癌细胞的浸润和增殖能力有关。

Cloning of liver cancer-related gene HCCA2 and association of that gene with liver cancer

Objective To study and clone novel liver cancer related genes and to explore the molecular basis of hepatocarcinogenesis. Methods mRNA differential display polymerase chain reaction (DDPCR) was used to clone several gene fractions that were highly expressed in hepatocarcinoma tissues and not expressed or lowly expressed in paracarcinoma tissue. A full length cDNA of one of such genes was obtained by screening the placenta cDNA library. The expression of this novel gene in 43 pairs of human hepatocellular carcinoma and paracarcinoma tissue was analyzed by Northern blotting. Results A full length cDNA of liver cancer related gene HCCA2 that was widely distributed in normal tissues and not expressed in liver tissue. The positive expression rate of this gene was 79% (34/43) in hepatocellular carcinoma tissue. Its expression was associated with the completeness of the tumor envelop and the expression of KI 67 PROTEIN ( P <0.01). Conclusion The novel gene HCCA2 may be related with the infiltration and proliferation of liver cancer.