肾综合征出血热病毒PS-6株和JR-C-1株抗原性的比较研究

目的 比较肾综合征出血热（HFRS）汉坦病毒（HV）PS-6株和JR-C-1株的抗原性，选择制备疫苗的毒种，方法 应用空斑减少中和试验（PRNT）和McAb间接免疫荧光试验（IFAT），检测PS-6株和JR-C-1株HFRSHV的抗原性。结果 PS-6和JR-C-1均为Ⅰ型HV。两株病毒的免疫血清对17株国内外不同来源的HV的中和反应和76-118株汉滩病毒（HTHV的免疫血清基本相似。但对一些病毒的中和反应效价两有差异。与多株McAb反应结果显示，两株病毒间存在抗原决定簇的差异。结论 PS-6和JR-C-1的抗原性与76-118株HTHV基本相同，PS-6和JR-C-1之间存在抗原性和抗原决定簇的差异。两合用抗原性会有互补作用。

Comparison of the Antigenicity of HFRS Virus Strain PS-6 and JR-C-1

Objective To compare the antigenicity of HFRS virus strain PS - 6 and JR - C - 1, selecting virus seed to prepare vaccine.Methods The antigenicity of PS - 6 and JR - C - 1 were detected using plague reduction cross neutralization test (PRNT) and indirect fluorescent antibody test (IFAT) .Result Both PS-6 and JR- C- 1 are HFRS virus type I .The neutralization reaction results of their immune serum to 17 strain HFRS viruses were basic resemblance with 76 -118 virus serum. But they were different in antibody titer from some viruses.The some epitope of PS - 6 and JR - C - 1 were different when they reacted with many McAb.Conclusions The antigenicity of PS-6, JR- C- 1 and 76- 118 are about same.PS- 6 is a few different from JR- C- 1 in antigenicity and epitope. The complementary effect will emerge when PS - 6 is used together with JR - C - 1.