Bcl-2蛋白、Ki-67抗原、雌激素受体及孕激素受体在子宫腺肌病子宫内膜中的表达

目的检测与分析细胞凋亡抑制因子(Bcl-2蛋白)、细胞核相关抗原(Ki-67抗原)、雌激素受体(ER)、孕激素受体(PR)在子宫腺肌病(AM)在位内膜、异位内膜和正常子宫内膜中的表达情况及相关性,探讨其在AM发病机制中的作用。方法采用免疫组织化学PicTureTM二步法检测40例AM在位内膜、异位内膜及38例正常子宫内膜中Bcl-2蛋白、Ki-67抗原、ER、PR的表达情况。采用Spearman等级相关分析这些因素间表达的相关性。结果(1)Bcl-2蛋白、Ki-67抗原在AM在位内膜和正常子宫内膜中表达有周期性变化,增生期高于分泌期(P<0.05);而在AM异位内膜中表达无明显周期性变化。(2)Bcl-2蛋白、Ki-67抗原在3种内膜的表达情况:AM异位内膜>AM在位内膜>正常子宫内膜,差异有统计学意义(P<0.05)。(3)AM在位内膜、正常子宫内膜中Bcl-2蛋白、Ki-67抗原的表达与ER、PR的表达呈正相关(P<0.05);AM异位内膜中Bcl-2蛋白、Ki-67抗原的表达与ER、PR的表达无相关。结论AM在位、异位内膜中Bcl-2蛋白、Ki-67抗原的异常表达可能与AM的发生、发展有关。

Expression of Bcl-2 protein,Ki-67 antigen,ER and PR in endometrium of human adenomyosis

Objective To examine the expression of cell apoptosis repressor factor,Bcl-2 protein,celluar nucleus related antigen,Ki-67 antigen,estrogen receptor(ER)and progesterone receptor(PR)in eutopic and ectopic endometrium of adenomyosis and normal endometrium,and explore the pathogenisis of adenomyosis.Methods PicTureTM immunohistochemical staining technique was used to examine Bcl-2 protein,Ki-67 antigen,ER and PR in 40 eutopic and ectopic endometrium of adenomyosis and 38 normal endometrium.The correlation among these factors was analyzed by Spearman.Results(1)The expression of Bcl-2 protein,Ki-67 antigen in eutopic endometrium of adenomyosis and normal endometrium showed obviously cyclic change.The expression level of them in proliferative stage was significantly higher than that in progestational stage(P<0.05).But in ectopic endometrium of adenomyosis,it showed no cyclic change.(2)The expression of Bcl-2 protein and Ki-67 antigen in ectopic endometrium of adenomyosis was significantly higher than that in eutopic endometrium of adenomyosis and normal endometrium(P<0.05),and it was higher in eutopic endometrium of adenomyosis than that in normal endometrium(P<0.05).(3)There was positive correlation of the expression of Bcl-2 protein and Ki-67 antigen with the expression of estrogen receptor and progesterone receptor in normal endometrium and eutopic endometrium of adenomyosis,but there was no correlation between them in ectopic endometrium of adenomyosis.Conclusion The abnormal expression of Bcl-2 protein and Ki-67 antigen in eutopic and ectopic endometrium of adenomyosis may be associated with pathogenisis of adenomyosis.