Ku80对人食管癌细胞放射敏感性及细胞周期的影响

目的 利用shRNA抑制Ku80蛋白的表达来研究Ku80在食管癌治疗中的作用。方法采用RNA干扰技术,构建shRNA-Ku80载体。用Western blot和RT-PCR方法证实RNA干扰的有效性和可行性。用克隆形成实验来研究shRNA抑制Ku80表达对γ线的敏感性。用流式分析法来研究shRNA抑制Ku80表达对细胞周期的影响。结果 成功构建了shRNA-Ku80载体。shRNA抑制Ku80表达增强食管癌细胞对γ线的敏感性。shRNA K3和H2使细胞周期阻滞于G₂/M期(61.8%与28.6%;64.3%与28.6%)。结论 shRNA抑制Ku80表达在食管癌治疗中有作用,有望成为肿瘤治疗的新靶点。

Effects of Ku80 on radiosensitivity and cell cycle in human esophageal carcinoma cells

Objective To study the role of Ku80 in the treatment of esophageal cancer through inhibiting the Ku80 expression by shRNA. Methods shRNA-Ku80 vector was constructed. The effectiveness and feasibility of RNA interference were confirmed by Western blot and RT-PCR methods. The cell sensitivity to gamma-rays was studied by colony formation assay. The effects of shRNA-Ku80 on cell cycle were observed by flow cytometry analysis. Results ShRNA-Ku80 vector was constructed successfully. Inhibition of Ku80 expression by shRNA enhanced the sensitivity of esophageal cancer cells to gamma-rays. shRNA K3 or shRNA H2 showed higher percentage of cells in G₂/M phase (61.8% vs 28.6%; 64.3% vs 28.6%). Conclusion Inhibitions of Ku80 expression by shRNA play a role in the treatment of esophageal cancer. Ku80 might be a new target of tumor treatment.