A rapid and simple one step method for isolation of poly(A)+ RNA from cells in monolayer. |
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| Authors: | K K Hartmann V Papa E J Brown U Doerries S M Rosenthal I D Goldfine |
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| Affiliation: | Diabetes and Endocrine Research Department, Mount Zion Hospital and Medical Center, San Francisco, CA 94120. |
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| Abstract: | A simple method was developed to isolate low abundance hormone receptor poly(A)+ RNA from cells in tissue culture. Adherent cells in tissue culture plates were directly released with proteinase K and solubilized in SDS. Oligo(dT) cellulose was directly added to the lysate to obtain poly(A)+ RNA. Yields and purity of the poly(A)+ RNA were comparable to other more lengthy methods. IGF-I receptor and insulin receptor mRNA could be detected on Northern blot without any degradation. |
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