转染神经生长因子基因的腹腔移植微囊化细胞对缺氧缺血性脑病新生大鼠脑损伤的保护作用

目的： 探讨腹腔移植微囊化神经生长因子(NGF)基因3T3细胞对缺氧缺血性脑病(HIBD)新生大鼠脑损伤的保护作用,为寻找治疗新生儿HIBD的有效方法提供实验依据。方法:新生Wistar大鼠60只,随机分为假手术组、空囊组和移植组，每组20只。假手术组大鼠腹腔注射生理盐水，空囊组大鼠制备HIBD模型后腹腔注射空微囊，移植组大鼠制备HIBD模型后腹腔注射微囊化3T3细胞。分别观察各组新生鼠脑组织水含量和海马区阳性细胞凋亡率。结果：空囊组和移植组大鼠存在不同程度脑水肿，空囊组大鼠脑组织含水量高于假手术组（P<0.05），移植组大鼠脑组织含水量与假手术组比较差异无统计学意义（P>0.05），但低于空囊组（P<0.05）。TUNLE免疫组织化学，空囊组与假手术组比较细胞凋亡率差异具有统计学意义（P<0.05），移植组阳性细胞凋亡率低于空囊(P<0.05）。结论：腹腔移植微囊化NGF基因3T3细胞可以减轻大鼠HIBD后脑水肿，且对大鼠HIBD后海马神经元有
保护作用。

Protective effect of celiac transplanting micro-capsulated cells transfected by nerve growing factor gene on hypoxic-ischemic brain damage in new-born rats

Objective To discuss the protective effect of celiac transplanting micro-capsulated 3T3 cells transfected by nerve growing factor(NGF) gene on hypoxic-ischemic brain damage(HIBD) in new-born rats,and to provide experimental basis for HIBD treatment.Methods 60 new-born Wistar rat,were randomly divided into sham operation group(n=20),blank capsule group(n=20),and transplant group(n=20).The rats in sham operation group were injected with saline.The rat models of HIBD were set up and used in blank capsule group and transplant group.The rats in blank capsule group were transplanted with blank capsules.The rats in transplant group were treated by transplanting micro-capsulated 3T3 cells transfected by NGF gene.The changes of water contents and the apoptotic rates of rat neuron in each group were analyzed.Results The rats in blank caspsule and transplant groups had various degrees of hydrocephalus,the water content in brain tissues of rats in blank capsule group was higher than that in sham operation group(P<0.05).The water content in brain tissues of rats in transplant group had no significant difference compared with sham operation group(P>0.05),but it was lower than that in blank capsule group(P<0.05).The TUNLE immunohistochemistry results showed that there was significant difference of apoptotic rate of neuron between blank capsule group and sham operation group(P<0.05),and the apoptotic rate of rat neuron in transplant group was lower than that in blank capsule group(P<0.05).Conclusion Celiac transplanting of micro-capsulated NGF 3T3 cells can relieve the hydrocephalus after HIBD and protect the hippocampal neuron after HIBD.