乙型肝炎患者HBV前C区A83点突变与临床关系

为探索ＨＢＶ前Ｃ区Ａ８３点突变与临床关系，采用错配ＰＣＲ（ｍｐ－ＰＣＲ）与限制性长度多态性分析（ＲＦＬＰ）相结合的方法，对６６例乙肝患者的血清标本，进行了ＨＢＶ前Ｃ区Ａ８３点突变的检测，结果：６６例患者中，３６例（５４．５％）检出Ａ８３点突变，慢肝中度，重度，肝炎肝硬变组明显高于ＨＢＶ携带者（Ｐ〈０．０５），抗－ＨＢｅ阳性组的检出率为７３．２％，明显高于ＨＢｅＡｇ阳性，而这些病例中９１．６％发生了

Correlation Between the variation of A83 at HBV preC Gene and Clinic in Patients with chronic Hepatitis B

To investigate the relationship between the HBV preC gene mutation and clinic,we detected the variation of A83 at HBV preC gene by the methods of mp PCR and RFLP in sera of 66 patients.The results showed that thirty of them (54 5%) had the preC A83 mutation and prevalence of the mutation of asymptomatic HBV carriers(ASC),mild chronic hepatitis B(CHB),moderate CHB,severe CHB and cirrhosis after hepatitis B was 12 5%,42 9%,65 4%,69 2% and 60 0% respectively.The rates of the preC A83 mutation in moderate CHB and severe CHB and cirrhosis after hepatitis B were higher than that in ASC(P<0 05)and they were also higher in patients with anti HBe positive than in patients with anti HBe negative.91 6% of the anti HBe positive patients were belong to chronic active liver diseases.The result sugges that the preC A83 mutation might be related to the long time inflammatory action of liver and to the immune pressure in infected hosts.