首页 | 本学科首页   官方微博 | 高级检索  
检索        

狼疮肾炎患者外周血单核细胞表达有功能的Fas
引用本文:刘郑荣,蒋建平,易朝辉,侯凡凡.狼疮肾炎患者外周血单核细胞表达有功能的Fas[J].中华肾脏病杂志,2002,18(1):14-16.
作者姓名:刘郑荣  蒋建平  易朝辉  侯凡凡
作者单位:510515,广州,第一军医大学南方医院肾内科中国人民解放军肾病中心
摘    要:目的 探讨狼疮肾炎(LN)患者单核细胞凋亡加速的机制。方法 对灯例LN患者进行观察及以 15例正常人为对照。采用流式细胞仪检测单核细胞 FaS和 Fas Ligand(FasL)的表达;单核细胞与重组人FaSL共孵育,碘化丙啶染色、流式细胞仪检测单核细胞凋亡,四甲基偶氮唑蓝(MTT)法观察单核细胞体外存活率。结果LN患者单核细胞Fas的表达较正常人明显上调(P<0.05);处于狼疮活动期的LN患者与狼疮静止期的LN患者之间Fas的表达差异无显著性意义(P>0.05)。正常人和LN患者的单核细胞表面均未检测出FasL的表达。单核细胞与重组人FasL在体外共同培养2h后,LN者单核细胞的凋亡较正常人明显增加(P<0.05),单核细胞与重组人 FasL在体外共同培养6h后,LN患者单核细胞的存活率较正常人明显低下(P<0.05)。结论 LN患者单核细胞表面功能性Fas表达上调是LN患者单核细胞凋亡加速的可能机制。

关 键 词:单核细胞  狼疮肾炎  Fas  FasLigand  外周血  细胞凋亡

Expression of functional Fas on monocytes in patients with lupus nephritis
LIU Zhengrong,JIANG Jianping,YI Zhanhui,et al..Expression of functional Fas on monocytes in patients with lupus nephritis[J].Chinese Journal of Nephrology,2002,18(1):14-16.
Authors:LIU Zhengrong  JIANG Jianping  YI Zhanhui  
Institution:LIU Zhengrong,JIANG Jianping,YI Zhanhui,et al. Renal Division,Nanfang Hospital,Guangzhou 510515,China
Abstract:Objective To elucidate the mechanism of accelerated apoptosis of monocytes in patients with lupus nephritis (LN). Methods 17 LN patients were enrolled in the study and 15 healthy volunteers were selected as controls. The expression of Faa and Fas ligand (FasL) was examined by immunofluorescent staining and flow cytometer analysis. The apoptosis and survival rate of monocytes cultured in vitro with human FasL (rHu-FasL) was analyzed by flow cytometer analysis and 5-diphenyl tetrazolium bromide (MTT) staining, respectively. Results Faa expression on monocytes was significantly higher in LN patients than that in healthy subjects. There was no difference of monocytes Fas expression between active LN patients and silent LN patients. There was no detectable FasL expression on monocytes from both LN patients and healthy controls. When monocytes cultured in vitro with rHu-FasL, there was a significantly increasing apoptosis and a decreasing survival rate of monocytes in LN patients as compared to healthy controls. Conclusion The up-regulated expression of functional Fas on monocytes may contribute to the accelerated apoptosis of monocytes in LN patients.
Keywords:Monocyte  Lupus nephritis  Fas  Fas ligand  Apoptosis  
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号