羊膜制备与保存中的微生物安全性研究

目的：探讨人羊膜在制备和保存过程中的微生物安全性及其控制,为建立羊膜库提供便捷、有效的微生物控制方法.方法：20只剖宫产胎盘的羊膜取材后,均按不同的无菌处理程序和不同保存方法分组,检测时段为保存后24h、1月、3月、12月,均作细菌、真菌、支原体检测.5只顺产胎盘的羊膜,作细菌、真菌、乳酸菌、支原体检测.随访提供羊膜的25名产妇6个月,复查血清学.结果：仅用无菌生理盐水浸泡组中,2只胎盘的羊膜出现细菌污染;20只剖宫产胎盘的羊膜在其余各组中均未查出微生物污染;1只顺产胎盘的羊膜出现乳酸菌污染.25名产妇6个月的血清学复查均为阴性.结论：来源于健康产妇剖宫产胎盘的羊膜经抗生素液规范化无菌处理后,现今常用的保存方法可基本保证羊膜在1年保存期内免受细菌、真菌和支原体的污染.

Study on microbial safety of HAM in preparation and preservation

Objective: To evaluate the microbial safety of human amniotic membrane(HAM) in preparation and preservation,and to establish effective methods of microbe control for HAM bank.Methods: Human amnions were collected from elective caesarean sections in 20 healthy women,and divided into groups based on different sterilization procedures and preservation methods.Bacteria,fungi and mycoplasmas were detected at 24h,1m,3m and 12m after preservation.Other amnions collected in eutocia from 5 healthy women were detected bacteria,fungi,lactobacilli and mycoplasmas.The series of serological determination for the parturients were followed up in 6 months.Results: Two HAM samples only soaked in NS were detected to be contaminated with E.coli and/or S.epidermidis,respectively from two caesarean sections.There is no microbe detected in all of the amnions from caesarean sections which were soaked in the antibiotics solution.One HAM sample from eutocia was yet detected to have lactobacilli after soak.The series of serological determination were all negative.Conclusion: During one year storage,HAM only from caesarean sections in healthy women,can successfully avoid contamination if soaked in the antibiotics solution then prepared and preserved with aseptic technique.