LFA-1与抗CD3 mAb共刺激对狼疮肾炎PBMC增殖和IgG合成的影响

目的 :探讨LFA 1与抗CD3mAb共刺激对狼疮肾炎 (LN)患者外周血单个核细胞 (PBMC)增殖与免疫球蛋白 (IgG)合成的影响。方法 :分离LN患者及健康人 (正常对照 )的PBMC ,采用Ficoll梯度密度离心法。细胞增殖实验采用3 H TdR掺入法。IgG测定采用ELISA法。结果 :在抗CD3mAb诱导下 ,2 9例LN非活动期和活动期PBMC中 ,3 H TdR的掺入量和IgG的合成均增加 ,且活动期的增殖强于非活动期 (P <0 .0 1) ;而单纯用抗CD3mAb对 12例正常对照的PBMC没有影响 (P >0 .0 5 )。抗CD3mAb与抗LFA 1mAb共刺激 ,均可增加LN患者 (活动期和非活动期 )及正常对照PBMC3 H TdR掺入量和IgG合成 ,其中活动期增殖效应强于非活动期 (P <0 .0 1) ,后者又强于正常对照 (P <0 .0 1)。抗LFA 1中和抗体的加入 ,可明显抑制LFA 1诱导的共刺激效应 (P <0 .0 1)。结论 :LFA 1促进LN患者PBMC增殖和IgG合成 ,可能是其参与LN发病的机制之一

Effects of LFA-1 costimulation on proliferation and IgG production of PBMCs from lupus nephritis patients

AIM: To study the effect of leukocyte function associated antigen 1(LFA 1) cos timulation on proliferation and immunoglobin production of peripheral blood mononuclear cells(PBMCs) form lupus nephritis(LN) patients. METHODS: 29 LN patients were enrolled in this study, and 12 healthy persons served as control. PBMCs from LN patients and healthy persons were obtained by Ficoll density gradient centrifugation, and celluar proliferation was detected by 3H TdR incorporation. IgG content in cultural supermatant was detected by ELISA. RESULTS: Stimulation of anti CD3 mAb alone could enhance the proliferation and IgG production of PBMCs from 29 LN patients,while the effects on PBMCs from patients in active phase were stronger than those from the patients in the inactive phase( P <0.01). But anti CD3 mAb had no influenence on PBMCs from healthy persons.The costimulation of anti CD3 mAb and LFA 1 could increase proliferation and IgG production of PBMCs from LN patients and healthy persons. The effects of this costimulation decreased in turn from active and inactive LN patients to normal persons( P <0.01). The costimulant effects of LFA 1 was inhibited by anti LFA 1 mAb. CONCLUSION: The effects of enhancing PBMC proliferation and IgG production by LFA 1 may be a mechanism of LN pathogenesis.