星形细胞肿瘤DEK的表达及其与病理分级的关系

目的:检测星形细胞肿瘤组织中DEK的表达，分析其与病理分级之间的关系。方法:应用免疫组化技术并用图像分析系统检测各级别星形细胞肿瘤组织及脑组织中DEK的表达水平。结果:各组免疫组化染色阳性率分别为:肿瘤组织（86.6%），其中毛细胞型星形细胞瘤(WHO I级66.7%)，弥漫性星形细胞瘤（WHO II级84.2%），间变性星形细胞瘤（WHO III级93.3%），胶质母细胞瘤（WHO IV级100.0%）；脑组织（33.3%）。各组免疫组化染色强度分别为:肿瘤组织 23.57±8.12，其中毛细胞型星形细胞瘤14.23±5.31，弥漫性星形细胞瘤19.52±6.68，间变性星形细胞瘤32.12±13.56，胶质母细胞瘤36.92±15.61；脑组织11.61±1.85。DEK在肿瘤组织中的表达显著高于其在脑组织中的表达（P<0.05）。结论:DEK的过表达与星形细胞肿瘤的发生密切相关，并且与肿瘤的病理分级也密切相关；DEK表达改变，为星形细胞肿瘤临床诊断、治疗和预后判断提供了新思路。

Study on relationship between expression of DEK in astrocytic tumors and its pathology classification

Objective:To detect the expression of DEK in astrocytic tumors and analyze the relationship between that and pathology classification of astrocytic tumors.Methods:Immunohistochemistry technique was adopted to examine the levels of expression of DEK protein in astrocytic tumors tissues and brain tissues and the system of image analysis was adopted to detect the results.Results:The positive rates of DEK protein in each group were:astrocytic tumors（86.6%），pilocytic astrocytoma （66.7%），diffuse astrocytoma（84.2%），anaplastic astrocytoma（93.3%），glioblastoma（100.0%），brain tissues（33.3%）.The expression of DEK protein in each group by immunohistochemical staining was:astrocytic tumors 23.57±8.12，pilocytic astrocytoma 14.23±5.31，diffuse astrocytoma 19.52±6.68，anaplastic astrocytoma 32.12±13.56，glioblastoma 36.92±15.61，brain tissues 11.61±1.85.The expression of DEK in astrocytic tumors was significantly higher than in brain tissues（P<0.05）.Conclusion:The over-expression of DEK has close relation with occurrence of astrocytic tumors.The expression of DEK provide a new way for clinic diagnosis，treatment and prognosis judgment of astrocytic tumors.