非小细胞肺癌胸水细胞块与组织学ALK检测的对比研究

目的:探讨非小细胞肺癌患者细胞学样本用于ALK基因断裂重组检测的临床价值。方法:收集具有组织学对照的细胞学样本102例，行免疫组化法，比较分析细胞块与组织块的ALK表达情况。免疫组化结果呈阳性的再行荧光原位杂交法检测ALK基因断裂重排情况。结果:组织块ALK免疫组化结果（+）、（++）、（+++）的共29例（阳性率为28.43%）；细胞块ALK免疫组化结果（+）、（++）、（+++）的共10例（阳性率为9.80%）。29例组织块和10例细胞块的ALK融合基因荧光原位杂交结果均阳性，荧光原位杂交结果与免疫组化结果一致（一致率为100%）。结论:细胞块ALK免疫组化的阳性检出率低于组织学结果，ALK荧光原位杂交结果与免疫组化结果一致。

Comparison study of pleural effusion cells and histological in ALK detection of NSCLC

Objective:To study the clinical value of ALK detection in the cytological samples of non-small cell lung cancer.Methods:All 102 cases of pleural effusion were used for immunohistochemical to test the ALK expression level in cell block samples and tissue samples respectively.Then,some IHC-positive cases were used to detect ALK gene on fluorescent in situ hybridization(FISH) method.Results:Tissue block ALK immunohistochemical results of "+","++" and "+++" were 29 cases,and the positive rate of histologic was 28.43%(29/102).Cell block ALK immunohistochemical results of "+","++" and "+++" were 10 cases,and the positive rate was 9.80%(10/102).ALK-FISH results of 29 positive tissue block cases and 10 positive cell block cases were positive as the same as the IHC.Conclusion:ALK immunohistochemical positive detection rate in cell blocks is lower than the histologic results.Also the ALK gene FISH detection is not different with IHC.