大肠癌细胞HCT-15肿瘤干细胞样细胞分离及成瘤性比较

目的:依据人大肠癌细胞HCT-15的表面标志物，分离其中的干细胞亚群。建立裸鼠体内原代大肠癌荷瘤模型，比较各亚群肿瘤体积和质量。方法:利用免疫磁珠分选技术，分离其中CD133/CD44干细胞亚群。分选得到CD133+CD44+、CD133+CD44-亚群分别接种于裸鼠体内，并观察肿瘤大小和质量。结果:CD133+CD44+和CD133+CD44-细胞亚群成功的从HCT-15细胞中分离出来。接种CD133+CD44+细胞的裸鼠形成的肿瘤体积［（2.76±0.22）cm3］和质量［（5.2±0.21）g］明显高于接种CD133+CD44-细胞裸鼠的肿瘤体积与质量［（1.56±0.34）cm3，（3.4±0.18）g］（P＜0.05）。结论:CD44阳性的大肠癌肿瘤干细胞成瘤能力明显高于CD44阴性的大肠癌肿瘤干细胞。

Isolation of cancer stem like cells from colorectal cancer cell HCT -15 and tumorigenicity comparison

Objective:According to the surface markers of HCT-15 cancer cell,to isolate the stem cancer cells.To build colorectal cancer xenografts models and compare the tumor size and weight of SCID hairless congenic mice.Methods:We isolated the stem cancer cells from human colorectal cancer cell HCT-15 by magnetic microbeads.CD133+CD44+ and CD133+CD44- stem cancer cells were subcutaneously inoculated to SCID hairless mice，then compared the tumor size and weight.Results:CD133+CD44+ and CD133+CD44- cancer stem cells were isolated successfully from HCT-15 cells.The tumor size derived from CD133+CD44+ cancer stem cells ［(2.76±0.22）cm3］ was larger than CD133+CD44- ones ［(1.56±0.34）cm3］(P＜0.05).And the weight ［(5.2±0.21）g］ was also higher than negative ones ［(3.4±0.18）g］(P＜0.05).Conclusion:The tumorigenicity of CD44 positive colorectal stem cancer cells was significantly higher than negative ones.