Vimentin在肾透明细胞癌中的表达及临床意义

目的:探讨波形蛋白(Vimentin)在人肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)和癌旁组织样本中的表达情况,并分析其与 ccRCC 临床分期、病理分级和侵袭转移等恶性生物学特性的关系及临床意义。方法:Western blot方法检测29例新鲜肾透明细胞癌及相应癌旁组织中Vimentin的表达；免疫组织化学方法检测120例肾透明细胞癌及相应癌旁组织中Vimentin的表达情况，分析Vimentin在肾透明细胞癌组织中及癌旁正常肾脏组织中的表达水平差异及Vimentin的表达与肿瘤大小、临床分期、组织病理分级、远处转移的关系。结果:Vimentin在肾透明细胞癌及相应癌旁组织中均有表达，前者的表达水平较后者显著升高（P＜0.01）；免疫组织化学显示肾透明细胞癌组织中，Vimentin的阳性表达率高达90.0%，与癌旁组织存在明显差异（P＜0.01）；Vimentin在肾透明细胞癌组织中以胞膜表达为主，其表达随肾癌的临床分期、肾包膜受侵、癌栓形成或远处转移有升高趋势，但组间比较经统计学分析均无显著性差异。结论:Vimentin在肾透明细胞癌组织中的表达明显高于癌旁正常肾组织，提示Vimentin在肾透明细胞癌的发生发展过程中发挥重要的作用。Vimentin在肾透明细胞癌组织中过表达，与肾癌的发展密切相关，可以作为肾细胞癌早期诊断、预后和复发评估的参考指标之一。

The expression and clinical significance of Vimentin in clear -cell renal cell carcinoma (ccRCC)

Objective:To study the expression of Vimentin in ccRCC and corresponding normal renal tissues,and the clinical significance.Methods:Western blot was used to detect the expression of Vimentin in 29 samples of fresh ccRCC and corresponding normal renal tissues.Vimentin expression was examined in 120 samples of paraffin-embedding ccRCC and the corresponding normal renal tissues using immunohistochemistry.Results:There was expression of Vimentin in both ccRCC and the corresponding normal renal tissues.In ccRCC tissues,the total levels of Vimentin proteins were significantly higher than that in the corresponding normal renal tissues (P＜0.01).The positive rate of Vimentin expression in immunohistochemistry was 90.0% in the ccRCC tissues,existing significant difference compared with the corresponding normal renal tissues (P＜0.01).Vimentin expression in renal clear cell carcinoma was not correlated with clinical stage,renal capsular invasion or distant metastases.Conclusion:The positive rate of Vimentin expression in renal cell clear cell carcinoma is higher than that in the adjacent normal kidney tissue,suggesting that the expression of vimentin may be correlated witht the occurrence of renal cell carcinoma.It may be important for the diagnosis of renal cell carcinoma.Overexpression of Vimentin in the ccRCC is closely correlated with the ccRCC development.It may play an important role in the process of ccRCC invasion and metastasis.