慢病毒载体介导RNA结合蛋白QKI-5过表达对前列腺癌细胞PC3增殖的抑制作用

目的:观察RNA结合蛋白Quaking-5（QKI-5）对雄激素非依赖性前列腺癌PC3细胞增殖的影响。方法:采用Western blot及逆转录-聚合酶链反应（RT-PCR）方法检测不同前列腺癌细胞株（PC3、LNCaP、DU145）及正常人前列腺上皮细胞（RWPE1）中QKI-5的表达水平；通过慢病毒感染并构建稳定过表达QKI-5的PC3细胞，噻唑蓝比色法（MTT法）绘制细胞生长曲线、流式细胞仪（FCM）检测细胞周期。 结果:3株前列腺癌细胞中QKI-5的表达水平明显低于正常前列腺上皮细胞，其中PC3细胞表达水平最低，细胞生长曲线显示稳定转染QKI-5的PC3细胞增殖能力明显低于对照组(P<0.05)，FCM检测发现稳定转染QKI-5的PC3细胞同对照组比较出现了明显的G1期阻滞（P<0.05）。 结论:RNA结合蛋白QKI-5可能与前列腺癌的发生发展有着密切的联系，慢病毒介导的QKI-5基因能够抑制PC3细胞的增殖。

Lentivirus-mediated RNA binding protein QKI-5 gene expression on the proliferation of PC3 cells

Objective:To study RNA binding protein QKI-5 on the proliferation of androgen independent prostate cancer PC3 cell line.Methods:The expression of QKI-5 protein and mRNA was detected by using Western blot and RT-PCR respectively.Recombinant lentivirus was used to infected prostate cancer cell line PC3.MTT assays were performed to determine cell proliferation.The changes in cell cycle were detected by flow cytometer after transfected.Results:The results of western blot and RT-PCR indicated that the three prostatic carcinoma cell lines had relatively lower QKI-5 mRNA and protein levels.PC3 cells had the lowest level among the three cell lines.After infected by lentivirus that it had been verified that the QKI-5 protein over-expression was identified cells infected with recombinant lentivirus.The MTT assay showed that QKI-5 could significantly inhibit PC3 cell proliferation（P<0.05).Compared with the blank control and Cherry group,Lv-QKI-5 group showed an increase in G1 phase(P<0.05).Conclusion:RNA-binding protein QKI-5 may be involved in the development of prostate cancer，lentiviral-mediated QKI-5 gene can inhibit the proliferation of PC3 cells.