ECT2 mRNA及蛋白在胃癌患者外周血中的表达及意义

目的:探讨ECT2 mRNA及蛋白在胃癌患者外周血中的表达水平及其意义。方法:应用逆转录聚合酶链式反应（RT-PCR）及酶联免疫吸附实验（ELISA）测定50例胃癌患者及30例健康志愿者外周血ECT2 mRNA及蛋白的表达水平。结果:胃癌患者外周血中ECT2 mRNA阳性表达率为54%，健康志愿者外周血中ECT2 mRNA均阴性表达(P<0.05)。ECT2蛋白在胃癌患者外周血中的浓度（1 306.389±215.824)ng/L显著高于健康志愿者外周血中的浓度（502.718±69.440)ng/L(P<0.05)，并且与TNM分期、淋巴结转移和浸润深度相关(P<0.05)。结论:外周血中ECT2 mRNA及蛋白的表达与胃癌关系密切，可作为反映胃癌发生、发展过程的有效分子指标。

Significance and the expression of ECT2 mRNA and protein in peripheral blood with gas-tric cancer

Objective:To explore the expression and significance of ECT2 mRNA and protein in peripheral blood of patients with gastric cancer.Methods:Using RT-PCR and ELISA to detect the mRNA and protein expression of ECT2 in peripheral blood from 50 patients with gastric cancer and 30 healthy volunteers.Results:ECT2 mRNA positive expression rate was 54% in peripheral blood of patients with gastric cancer and 0% in controls（P<0.05）.ECT2 concentration in peripheral blood of patients with gastric cancer (1 306.389±215.824)ng/L was significantly higher than that in peripheral blood of healthy volunteers (502.718±69.440)ng/L (P<0.05), and associated with TNM stage, lymph node metastasis and invasion depth (P<0.05).Conclusion:The expression of ECT2 mRNA and protein in peripheral blood significantly correlated with gastric cancer progression, and can be as an effective molecular marker for gastric cancer.