肿瘤坏死因子相关凋亡诱导配体对非小细胞肺癌细胞凋亡诱导的基础研究

目的:观察肿瘤坏死因子相关凋亡诱导配体对非小细胞肺癌小鼠模型肿瘤细胞凋亡诱导的作用。方法:30只小鼠接种浓度为5×106/ml非小细胞肺癌细胞Tc-1。荷瘤小鼠随机分为6组（空白对照组和5种浓度rhTRAIL实验组）。空白对照组每天注射一次灭菌生理盐水，实验组每天注射浓度不同的rhTRAIL溶液，各组均连续注射15d。每2d测量小鼠肿瘤长短直径，计算肿瘤体积。取小鼠脾脏细胞培养，测量培养液上清TNF-α浓度。结果:rhTRAIL给药组小鼠肿瘤体积明显低于空白对照组（P<0.05），随着rhTRAIL浓度的增加，肿瘤体积相应减小；小鼠的Tc-1细胞凋亡率随着rhTRAIL浓度增高，其凋亡率逐渐升高；rhTRAIL给药组小鼠的TNF-α浓度均明显高于空白对照组，组间差异具有显著性（P<0.05）。结论:rhTRAIL能有效增强非小细胞肺癌小鼠体内的细胞因子分泌，诱导荷瘤小鼠的肿瘤细胞凋亡，表明rhTRAIL对非小细胞肺癌小鼠的肿瘤细胞具有一定的抑制作用。

Experimental study of tumor necrosis factor - related apoptosis - inducing ligand in non-small cell lung cancer cell apoptosis induced

Objective:To observe the effect of tumor necrosis factor-related apoptosis-inducing ligand on non-small cell lung cancer mouse model of tumor cell apoptosis.Methods:Thirty mice were inoculated with non-small cell lung cancer cells，seeding concentration 5×106 Tc-1 cells.30 tumor-bearing mice were randomly divided into six groups (control group and five concentrations rhTRAIL positive experimental groups).The control group was injected with NS，the positive control group was injected with solution of different concentrations rhTRAIL every day，each group of continuous injection 15d，length of the diameter of the tumor，tumor volume was calculated.Mouse spleen cells were measured for TNF-α concentrations and compared.Results:In five positive tumor volume was significantly lower than the control group (P<0.05)；in 5 positive control groups rhTRAIL concentration increased，the tumor volume was reduced accordingly；in Tc- five groups positive rate of apoptosis rhTRAIL concentration increased，the apoptosis rate increased gradually；in 5 positive groups TNF-α concentrations were significantly higher than the control group，the difference was significant (P<0.05).Conclusion:rhTRAIL can effectively enhance the non-small cell lung cancer in mice secrete cytokines，effectively induce tumor cell non-small cell lung cancer in mice apoptosis.rhTRAIL can inhibit non small cell lung cancer cells in mice with a certain extent.