外源性Rb基因对U937细胞泡沫化过程中CD36表达的影响

为研究抑癌基因Rb基因在U937细胞泡沫化过程中的表达，应用重组腺病毒载体导入外源性Rb基因。采用逆转录聚合酶链反应和流式细胞术检测氧化型低密度脂蛋白致U937细胞泡沫化过程中清道夫受体CD36和Rb基因的表达和外源性Rb基因导入U937细胞后CD36和Rb基因的表达。结果发现，氧化型低密度脂蛋白致U937细胞泡沫化过程中，Rb mRNA和蛋白的表达随作用时间延长呈下调趋势，CD36 mRNA和蛋白的表达则呈上调趋势；随着外源性Rb导入U937细胞并表达，CD36表达呈下调趋势。结果提示，外源性Rb基因的导入可以下调清道夫受体CD36的表达。

Effect of Exogenous Rb Gene on Expression of CD36 Gene in U937 Cells Treated with Oxidized Low Density Lipoprotein

Aim To study the expression level of retinoblastoma (Rb) and CD36 in U937 cells treated with oxidized low density lipoprotein(ox-LDL) or pretreated with exogenous Rb gene. Methods Human myeloid leukemia U937 cells were incubated with ox-LDL(80 mg/L) at 0 h,12 h,24 h and 48 h after ox-LDL loaded respectively. CD36 and Rb gene expression were detected by RT-PCR and flow cytometric analysis(FCA). Results RT-PCR and FCA results showed that the expression level of Rb was downregulated and the expression level of CD36 upregulated from 0h to 48h induced by ox-LDL. While after being transfected by exogenous Rb gene via a recombinant adenovirus vector, the expression level of Rb was upregulated and the expression level of CD36 downregulated with a time-dependent manner. Conclusions Ox-LDL could downregulate the expression level of Rb mRNA and protein, and upregulate that of CD36. Introduction of exogenous Rb gene could downregulate expression of CD36 of U937 cells.