| TLR2、TLR4、TLR5和TLR9在小鼠Hp感染模型中的表达 |
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| 引用本文： | 刘翔,林漫鹏,吕建忠,林虹.TLR2、TLR4、TLR5和TLR9在小鼠Hp感染模型中的表达[J].实用医学杂志,2011,27(13):2327-2329. |
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| 作者姓名： | 刘翔 林漫鹏 吕建忠 林虹 |
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| 作者单位： | 广州医学院第二附属医院消化内科,510260 |
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| 基金项目： | 广东高校优秀青年创新人才培养计划项目 |
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| 摘 要： | 目的:研究BALB/c小鼠在幽门螺杆菌(Hp)感染前后和用抗生素治疗后胃黏膜组织中Toll样受体(TLR)2、TLR4、TLR5、TLR9、IL-1β的表达,探讨Toll样受体家族在Hp感染机制中的作用。方法:60只BALB/c小鼠随机分为3组,第1组不予处理(正常组),第2组予感染Hp(Hp感染组),第3组感染Hp后予抗生素治疗(抗生素治疗组)。RT-PCR和Western blotting法半定量检测小鼠胃内TLR2、TLR4、TLR5、TLR9、IL-1β的表达,Giemsa染色切片计数Hp定植数量,HE染色切片判断黏膜炎症水平。结果:(1)TLR5、TLR9在各组小鼠胃黏膜组织中均无表达。(2)TLR2在Hp感染组胃黏膜组织中表达(PCR:0.13±0.025;Western:1.32±0.27)高于抗生素治疗组(PCR:0.04±0.011;Western:0.43±0.08),正常组无表达。(3)TLR4在Hp感染组胃黏膜组织中表达(PCR:0.22±0.051;Western:0.72±0.17)高于抗生素治疗组(PCR:0.06±0.009;Western:0.21±0.04),正常组无表达。(4)IL-1β在Hp感染组胃黏膜组织中有表达(PCR:0.27±0.038;Western:0.58±0.14),抗生素治疗组和正常组无表达。结论:TLR2、TLR4可能参与了Hp的致病机制,TLR5、TLR9可能未参与Hp的致病机制。
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| 关 键 词： | 螺杆菌 幽门 TLR2 TLR4 TLR5 TLR9 IL-1β |
Expression of TLR2, TLR4, TLR5 and TLR9 in Hp infected mic |
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| eLIU Xiang,LIN Man-peng,LV Jian-zhong,LIN Hong.Expression of TLR2, TLR4, TLR5 and TLR9 in Hp infected mic[J].The Journal of Practical Medicine,2011,27(13):2327-2329. |
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| Authors: | eLIU Xiang LIN Man-peng LV Jian-zhong LIN Hong |
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| Institution: | LIU Xiang,LIN Man-peng,LV Jian-zhong,LIN Hong. The Second Affiliated Hospital of Guangzhou Medical College,Guangzhou 510260,China |
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| Abstract: | Objective To investigate the expression of TLR2,TLR4,TLR5 and TLR9 in gastric mucosa of BALB/c mice,which were before and after Hp infection and after treatment by antibiotics in gastric mucosa of BALB/c mice,and to explore the role of TLR family in Hp infection. Methods 60 BALB/c mice were randomly divided into 3 groups,Group 1 untreated(normal group) ,Group 2 infected with Hp(Hp infected group) ,Group 3 treated by antibiotics after infected with Hp(antijbiofic treatment group) . RT-PCR and Western blottin... |
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| Keywords: | TLR2 TLR4 TLR5 TLR9 |
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