| 吴茱萸致小鼠肝毒性分子机制研究 |
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| 引用本文： | 廖文强,李波,李莉,赵军宁. 吴茱萸致小鼠肝毒性分子机制研究[J]. 中国中药杂志, 2014, 39(24): 4865-4868 |
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| 作者姓名： | 廖文强 李波 李莉 赵军宁 |
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| 作者单位： | 四川省中医药科学院, 四川 成都 610041;四川省中医药科学院, 四川 成都 610041;四川省郫县人民医院, 四川 郫县 611730;四川省中医药科学院, 四川 成都 610041;四川省中医药科学院, 四川 成都 610041 |
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| 基金项目： | 国家重点基础研究发展计划(973)项目(2009CB522801);国家自然科学基金面上项目(81073047);四川省科技厅基本科研业务专项(A-2011N-22) |
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| 摘 要： | 目的:研究吴茱萸提取物致小鼠肝毒性的分子机制。 方法:30只KM小鼠随机分为3组, 连续灌胃给予吴茱萸提取物15 d后, Western blot法检测小鼠肝脏 Erk1/2, CDK8, CK1e, Stat3, Src蛋白表达量。 结果:吴茱萸提取物导致Erk1/2, CDK8, CK1e表达量上调(P <0.01), Stat3, Src表达量下调(P <0.01)。 结论:吴茱萸致肝毒性分子机制可能与Erk1/2, CDK8, CK1e, Stat3和Src信号分子相关。
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| 关 键 词： | 吴茱萸 肝毒性 分子机制 Western blot |
| 收稿时间： | 2014-05-24 |
Study on molecular mechanism of Euodiae Fructus on liver toxicity in MICE |
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| LIAO Wen-qiang,LI Bo,LI Li and ZHAO Jun-ning. Study on molecular mechanism of Euodiae Fructus on liver toxicity in MICE[J]. China Journal of Chinese Materia Medica, 2014, 39(24): 4865-4868 |
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| Authors: | LIAO Wen-qiang LI Bo LI Li ZHAO Jun-ning |
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| Affiliation: | Sichuan Academy of Traditional Chinese Medicine Sciences, Chengdu 610041, China;Sichuan Academy of Traditional Chinese Medicine Sciences, Chengdu 610041, China;Pixian County People's Hospital, Pixian 611730, China;Sichuan Academy of Traditional Chinese Medicine Sciences, Chengdu 610041, China;Sichuan Academy of Traditional Chinese Medicine Sciences, Chengdu 610041, China |
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| Abstract: | Objective:To study the molecular mechanism of extracts from Euodia rutaecarpa on hepatotoxicity in mice. Method:Totally 30 KM mice were divided into 3 groups and orally administrated extracts from E. rutaecarpa for consecutively 15 days. The expressions of Erk1/2, CDK8, CK1e, Stat3 and Src were detected by Western blotting method. Result:The extracts from E. rutaecarpa could up-regulated Erk1/2, CDK8 and CK1e expressions (P <0.01) and down-regulate Stat3 and Src (P <0.01). Conclusion:The molecular mechanism of E. rutaecarpa on hepatotoxicity may be correlated with Erk1/2, CDK8, CK1e, Stat3 and Src signal molecules. |
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| Keywords: | Euodia rutaecarpa hepatotoxicity molecular mechanism Western blotting |
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