错配修复基因hMLH1、hMSH2及p53在肺癌组织中的表达及意义

目的探讨人类错配修复基因hMLH1、hMSH2及D53在肺癌组织中的表达及意义。方法运用免疫组织化学sP法对56例肺癌组织中hMLH1、hMSH2及p53的表达进行检测。结果56例肺癌组织中hMLH1的阳性表达率为35％，hMSH2阳性表达率为28．6％，分化程度高者阳性率显著高于分化程度低者（P〈0．01），有淋巴结转移者hMLH1及hMSH2阳性率低于无淋巴结转移者（P〈0．05），不同病理组织学类型之间hMLH1及hMSH2表达无显著差别（P〉0．05）；56例肺癌组织中，p53阳性率为51．8％，分化程度低者p53阳性率高于分化程度高者（P〈0．05），有淋巴结转移者p53阳性率高于无淋巴结转移者（P〈0．01），不同病理组织学类型之间p53阳性率无差别（P〉0．05）；hMLHI及hMSH2阴性表达者的p53阳性率高于hMLH1及hMSH2阳性表达者（P〈0．05）。结论hMIH1及hMSH2基因的缺陷及p53的表达与肺癌的发生发展过程并与分化程度及有否淋巴结转移有关。

Expression and its significance of human mismatch repair gene (hMLH1/hMSH2) and p53 in lung cancer patients

Objective To investigate the expression of human mismatch repair gene(hMLH1/hMSH2)and p53 in lung cancer patients and its significance.Methods Expression of hMLH1/hMSH2 and p53 in 56 lung cancer patients was determined by S-P immunohistochemical staining.Results The positive expression rates of hMLH1 and hMSH2 in 56 lung cancer cases were 35% and 28.6% respectively.The positive rates of expression of hMLH1/hMSH2 in the patients at high and moderate differentiation stages were significantly higher than those of the others(P<0.01).Meanwhile,the positive rate of cases with lymph node metastasis was lower than that of the patients without lymph node metastasis(P<0.05).It wasn't positively correlated with pathological types.The positive expression rate of p53 in 56 lung cancer cases was 51.8% and in the patients at high and moderate differentiation stages were lower than those of others(P<0.01).The rate in patients with lymph node metastasis was significantly higher than that of patients without metastasis(P<0.05).The rate of p53 in hMLH1/hMSH2 positive lung cancer tissue was higher than that in hMLH1/hMSH2 negative tissue(P<0.05).Conclusion The abnormal expression of hMLH1/hMSH2 and p53 might be involved in the carcinogenesis of lung cancer and correlated with differentiation stages and lymph node metastasis.