甘味颗粒对小鼠急性应激诱导行为绝望抑郁的改善作用及其机制研究

目的考察甘味颗粒对急性应激诱导行为绝望抑郁小鼠行为的影响,并探讨其作用机制。方法 ICR小鼠随机分为对照组、模型组、逍遥丸组、氟西汀组以及甘味颗粒2.11、6.33、19.00 g/kg组,每组15只。模型组和对照组均ig给予蒸馏水,逍遥丸组ig给予逍遥丸1.50 g/kg,氟西汀组ig给予盐酸氟西汀分散片3.3 mg/kg,甘味颗粒组ig给予甘味颗粒2.11、6.33、19.00 g/kg。1次/d,连续给药10 d。各组小鼠悬尾5 min和常温强迫游泳6 min进行急性应激诱导行为绝望抑郁模型,观察应激小鼠的行为学效应。采用酶联免疫吸附(ELISA)法检测小鼠血浆中皮质醇水平以及脑组织中单胺类神经递质5-羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)水平。结果与模型组比较,甘味颗粒6.33、19.00 g/kg组的悬尾不动时间显著减少,差异具有统计学意义(P0.05、0.01);甘味颗粒2.11、6.33、19.00 g/kg组的强迫游泳不动时间显著减少,差异具有统计学意义(P0.05、0.01)。与模型组比较,甘味颗粒6.33、19.00 g/kg组血浆皮质醇水平均明显降低,差异具有统计学意义(P0.05)。与模型组比较,甘味颗粒6.33、19.00 g/kg组5-HT水平明显升高,差异具有统计学意义(P0.05、0.01),甘味颗粒2.11、6.33、19.00 g/kg组DA、NE水平显著升高,差异具有统计学意义(P0.05、0.01)。结论甘味颗粒对急性应激诱导行为绝望抑郁小鼠行为具有显著的改善作用,可能是通过升高单胺类神经递质5-HT、DA、NE水平,降低血浆皮质醇水平而发挥其抗抑郁作用。

Improvement of Ganwei Granules on mice with behavioral despair and depression induced by acute stress and its mechanism

Objective To investigate the effect of Ganwei Granules on mice with behavioral despair and depression induced by acute stress and explore its mechanism. Methods ICR mice were randomly divided into control group, model group, Xiaoyao Pills group, fluoxetine group, and Ganwei Granules (2.11, 6.33, and 19.00 g/kg) groups, and each group had 15 mice. Mice in control and model groups were ig administered with distilled water, mice in Xiaoyao Pills group were ig administered with Xiaoyao Pills 1.50 g/kg, and mice in fluoxetine group were ig administered with Fluoxetine Hydrochloride Dispersible Tablets 3.3 mg/kg. Mice in Ganwei Granules groups were ig administered with Ganwei Granules 2.11, 6.33, and 19.00 g/kg, respectively. All animals were ig administered once daily, and treated for 10 d. Behavioral despair and depression model induced by acute stress was built by tail suspension for 5 min test and forced swimming for 6 min test. The behavioral effects were observed. The levels of cortisol in plasma and monoamine neurotransmitter serotonin (5-HT), norepinephrine (NE), dopamine (DA) in brain were determined by enzyme-linked immunosorbent assay (ELISA). Results Compared with the model group, the fixed time of tail suspension in Ganwei Granules 6.33 and 19.00 g/kg groups were significantly reduced, and there were differences between two groups (P < 0.05, 0.01), while the fixed time of forced swimming in Ganwei Granules 2.11, 6.33, and 19.00 g/kg groups were significantly reduced, with significant differences between two groups (P < 0.05, 0.01). Compared with the model group, the levels of cortisol in plasma of Ganwei Granules 6.33 and 19.00 g/kg groups were significantly decreased, with significant differences between two groups (P < 0.05). Compared with the model group, the levels of 5-HT in plasma of Ganwei Granules 6.33, 19.00 g/kg groups were significantly increased, and the levels of DA and NE in plasma of Ganwei Granules 2.11, 6.33, and 19.00 g/kg groups were also obviously increased, and there were differences between two groups (P < 0.05, 0.01). Conclusion Ganwei Granules have significant improved effects on mice with behavioral despair and depression induced by acute stress, which may be related to the increase of monoamine neurotransmitter 5-HT, DA, and NE, and the reduction of cortisol in plasma.