| 非霍奇金病t(14;18)易位的PCR检测 |
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| 引用本文： | 王彼得,钟玉萍,王玲,张春玲,吴弘英. 非霍奇金病t(14;18)易位的PCR检测[J]. 青岛大学医学院学报, 2000, 36(1): 29-30 |
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| 作者姓名： | 王彼得 钟玉萍 王玲 张春玲 吴弘英 |
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| 作者单位： | 1. 青岛大学医学院第二附属医院血液科,青岛,266042
2. 青岛市市立医院血液科 |
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| 摘 要： | ①目的 检测非霍奇金病的微小残留病。②方法 徼增琼脂糖凝胶电泳检测法，测定５０例非霍奇金病ｂｃｌ－２基因表达与ｔ（１４；１８）染色体易位的关系。③结果 在这个易位中，位于１８ｑ＾２１上ｂｃｌ－２原癌基因与１４ｑ＾３２上的ＩｇＨ基因两便，使ｂｃｌ－２基因表达失常。本组５０例淋巴瘤病人中，１１例（２２％）表达ｔ（１４；１８）易位，５例经过治疗已获得缓解的病人中，１例检测到ｔ（１４．１８）易位随访９０ｄ
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| 关 键 词： | 非霍奇金病 聚合酶链反应 t（14 18）易位 bcl－2 |
| 文章编号： | 1001-4047(2000)01-0029-02 |
| 修稿时间： | 1999-05-15 |
DETECTION OF t(14; 18) TRANSLOCATION IN NON-HODYKIN BY POLYMERASE CHAIN REACTION (PCR) |
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| WANG Bide,ZHONG Yuping,WANG Ling,et al The. DETECTION OF t(14; 18) TRANSLOCATION IN NON-HODYKIN BY POLYMERASE CHAIN REACTION (PCR)[J]. Acta Academiae Medicinae Qingdao Universitatis, 2000, 36(1): 29-30 |
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| Authors: | WANG Bide ZHONG Yuping WANG Ling et al The |
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| Affiliation: | WANG Bide,ZHONG Yuping,WANG Ling,et al The Department of Hematology,The Second Affiliated Hospital of Qingdao University Medical College,Qingdao 266042, [ |
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| Abstract: | Objective To detect minimal residual disease of non Hodykin lymphoma.MethodsThe relationship between the expression of bcl 2 gene and the chromosome translocation of t(14;18) was measured by polymerase chain reaction(PCR).Results In the translocation, the juxtaposition of the proto oncogene bcl 2 at chromosome segment 18q 21 and the Ig heavy chain locus at 14q 32 resulted in irregular expression of bcl 2. 50 patients with lymphomas were studied by polymerase chain reaction. 11 cases showed t(14;18) translocation (22%). t(14;18) translocation was detected in 1 of 5 cases in catabasis after treatment and this patient relapsed after three months.Conclusiont(14;18) translocation may be a remarkable molecular marker for transition of cells or progress of lymphomas and has a very important value for detecting minimal residual disease(MRD). [ |
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| Keywords: | lymphoma non Hodgkin's minimal resedeual disease apoptosis lymphoma chromosomes polymerase chain reaction |
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