RA患者外周血CD4/CD8 T细胞比值及CD4+ CD25+ T细胞的检测及其临床意义

目的 观察活动期类风湿性关节炎（RA）患者外周血T细胞CD4／CD8比值及CD4^＋ CD25^＋T细胞（Tn细胞）的数量，探讨其在RA诊治中的价值。方法 用流式细胞术检测CD4^＋ CD25^＋T细胞及TR细胞的数量。结果 49例急性期RA患者的CD4／CD8比值和TR细胞数量显著高于正常人群组。28例RA患者治疗后的TR细胞数量显著高于治疗前，而CD4／CD8比值的变化无统计学意义。结论 RA治疗后症状改善患者，TR细胞数量明显上升，RA的发病和发展与该细胞数量密切相关。

Study on ratio of CD4/CD8 and the number of CD4 + CD25 + Treg cells in PBMC of rheumatoid arthritis patients

Objective To detect the ratio of CD4+ and CD8+ T cells and the number of CD4+CD25+ T regulatory cells(Treg)in human peripheral blood mononuclear cells(PBMC)of rheumatoid arthritis(RA)patients and evaluate the significance for diagnosis of RA.Methods The number of CD4+,CD8+ T cells and Treg cells were detected by flow cytometry and the ratio of CD4/CD8 was calculated.Results The ratio of CD4/CD8 and the number of CD4+CD25+ T cells detected in 49 RA patients were significantly higher than that in health group with statistical significance(P<0.05,P<0.01).The number of Treg cells in 28 RA patients after therapy was significantly higher than that before therapy(P<0.01),while no significant change of CD4/CD8 ratio.Conclusion The number of Treg cells increased significantly in RA patients whose symptoms were moderated.We think the number of Treg cells may closely related to the occurrence and development of RA.