士的宁敏感的甘氨酸受体对吸入麻醉药催眠和镇痛作用的影响

目的探讨士的宁(strychnine,Stry)敏感的甘氨酸受体(strychnine-sensitive glycine receptor,GlyR)与吸入麻醉药异氟烷、恩氟烷、七氟烷和乙醚催眠、镇痛作用的关系。方法建立小鼠腹腔注射吸入麻醉药催眠、镇痛模型,在催醒和热板实验中分别观察侧脑室(intracerebroventricular,icv)或鞘内(intrathecal,it)注射不同剂量士的宁对小鼠睡眠时间(sleeping time,ST)和热板疼痛指数(pain index in hot-platetest,HPPI)的影响。结果催醒实验中,士的宁1、2、4μg icv对上述4种吸入麻醉药的ST均无影响(P>0.05);热板实验中,士的宁0.1、0.2、0.4μg it能够剂量依赖性地减少吸入麻醉药恩氟烷、七氟烷和乙醚镇痛小鼠的HPPI(P<0.05,P<0.01);士的宁0.1μg it对异氟烷镇痛小鼠的HPPI没有影响(P>0.05),0.2、0.4μg it可减少异氟烷镇痛小鼠的HPPI(P<0.05,P<0.01)。结论士的宁敏感的甘氨酸受体是吸入麻醉药异氟烷、恩氟烷、七氟烷和乙醚镇痛作用的重要靶位,但与其催眠作用关系不大。

Effects of strychnine-sensitive glycine receptor on the hypnotic and analgesic effects of inhalation anesthetics

Aim To investigate the relationship between strychnine-sensitive glycine receptor (GlyR) and the hypnotic and analgesic effects of aether,enflurane, isoflurane, sevoflurane. Methods After having established the mice model of hypnosis and analgesia by intraperitoneally injecting (ip) appropriate doses of aether,enflurane, isoflurane or sevoflurane, we intracerebroventricularly (icv) or intrathecally (it) injected different doses of strychnine and observed effects on the sleeping time (ST) by using awaken test and the pain index in hot-plate test (HPPI) by using hot-plate test. Results In awaken test,strychnine 1,2,4 μg (icv) have no distinctive effect on the ST of mice treated with four inhalation anesthetics mentioned above (P>0.05); in hot-plate test , strychnine at 0.1,0.2,0.4 μg (it) can significantly and dose dependently decrease the HPPI of the mice treated with aether,enflurane and sevoflurane (P<0.05,P<0.01); strychnine at 0.1 μg (it) did not affect the HPPI of mice treated with isoflurane(P>0.05), but at 0.2,0.4 μg (it) could significantly decrease the HPPI of mice treated with isoflurane(P<0.05,P<0.01).Conclusion GlyR may be important targets for the analgesic effects of aether ,enflurane, isoflurane and sevoflurane,but have no relationship with their hypnotic effects.