| pH敏感多肽修饰载紫杉醇脂质体的制备及评价 |
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| 引用本文： | 龚锐,李胜英,黎云燕. pH敏感多肽修饰载紫杉醇脂质体的制备及评价[J]. 华西药学杂志, 2015, 30(2): 134-136 |
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| 作者姓名： | 龚锐 李胜英 黎云燕 |
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| 作者单位： | 1. 天津医科大学第二医院药学部,天津,300070
2. 天津儿童医院检验科,天津,300070 |
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| 摘 要： | 目的制备p H敏感多肽修饰载紫杉醇脂质体(p HS-LP-PTX),并评价其体外性质。方法采用薄膜分散法制备PHS-LP-PTX,MTT实验考察脂质体对MCF-7细胞和Hep G2细胞的毒性,通过细胞摄取实验考察脂质体与肿瘤细胞的结合能力。结果所制备的p HS-LP-PTX在p H6.4时平均粒径为125.5±13.4 nm,Zeta电位为10.47±2.53 m V,24 h内具有良好的血清稳定性。MTT结果显示:p H6.4时,p HS-LP-PTX的细胞毒性优于各对照组。体外细胞摄取实验表明:p H6.4时,MCF-7细胞和Hep G2细胞对p HS-LP-PTX的摄取效率优于p H7.4时和普通脂质体。结论紫杉醇脂质体经过p H敏感多肽修饰后,能增强脂质体在酸性环境下的细胞穿透能力,是一种良好的p H敏感型肿瘤靶向给药系统。
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| 关 键 词： | pH敏感 紫杉醇 肿瘤靶向 脂质体 多肽修饰 薄膜分散法 细胞摄取 |
Study on the preparation and properties of pH sensitive peptide modified paclitaxel loaded liposome in vitro |
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| GONG Rui,LI Sheng-ying,LI Yun-yan. Study on the preparation and properties of pH sensitive peptide modified paclitaxel loaded liposome in vitro[J]. West China Journal of Pharmaceutical Sciences, 2015, 30(2): 134-136 |
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| Authors: | GONG Rui LI Sheng-ying LI Yun-yan |
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| Affiliation: | GONG Rui;LI Sheng-ying;LI Yun-yan;Department of Pharmacy,the Second Hospital of Tianjin Medical University;Tianjin Children’s Hospital Clinical Laboratory; |
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| Abstract: | |
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| Keywords: | pH sensitive Paclitaxel Tumor targeting Liposomes Peptide modified Thin film hydration method Demonstrated |
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