海南岛两株甲病毒基因组3＇末端核苷酸序列的克隆与分析

目的 从分子水平鉴定海南省分离的两株甲病毒（ＨＢｂ１７和Ｍ１病毒）。方法 采用ＲＴ－ＰＣＲ方法，分别扩增两株病毒基因组的３’末端核苷酸序列，扩增产物经亚克隆，筛选重组子并测定核苷酸序列对序列进行分析。结果 ＨＢｂ１７和Ｍ１病毒分别扩增出约１．６ｋｂ和１．３ｋｂ的特异片段。序列分析表明，在３’末端非翻译区ＨＢｂ１７病毒和Ｍ１病毒与罗斯病毒（国际标准株Ｔ４８病毒）核苷酸同源性为９９％，Ｍ１病毒与鹭山病

Cloning and prinary analysis of 3'end genome of two alphaviruses isolated from Haiman Province of China

OBJECTIVE: To further characterize the HBbl7 and Ml viruses isolated from Hainan Province by molecular biology. METHODS: The fragment containing partial El and 3'untranslated region of each of the viral isolate was amplified by RT-PCR, then subcloned. Recombinant was screened and sequenced. Analysis and comparison were carried out. RESULTS: 1.6kb fragment was amplified from HBbl7 and a 1.3kb was from Ml Comparison of sequences showed, in 3'untranslated region the nucleotide homology between HBbl7 and T48, the prototype of Ross River virus, is 99%, between Ml and Sagiyama virus is 98%. In the partial El sequence, the nucleotide (amino acid) homology between HBbl7 and T48 is 99%(99%),between Ml and Sagiyama virus is 97%(99%), and between Ml and Cetah virus is 94% (98%). CONCLUSIONS: Sequence analysis showed HBb17 virus belongs to Ross River virus, Ml virus belongs to Sagiyama virus or Cetah virus.