| 正交设计优选超声提取蜘蛛香中缬草素的提取工艺 |
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| 引用本文： | 陈玉娟,石晋丽,闫兴丽,刘勇,肖培根. 正交设计优选超声提取蜘蛛香中缬草素的提取工艺[J]. 世界科学技术, 2009, 11(6): 889-891 |
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| 作者姓名： | 陈玉娟 石晋丽 闫兴丽 刘勇 肖培根 |
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| 作者单位： | 北京中医药大学中药学院,北京,100102 |
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| 基金项目： | 国家自然科学基金项目(30572463):菌药蜘蛛香治疗肠激综合征(IBS)的物质基础及作用机理研究 |
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| 摘 要： | 目的：研究超声波提取蜘蛛香中缬草素的最佳工艺。方法：采用4因素3水平L9（3^4）正交试验法，以缬草素的得率为考察指标，对超声波提取蜘蛛香中缬草素的工艺进行优化。在优选出的超声波最佳提取工艺条件下，将该法与渗漉法进行比较。结果：优选的超声波提取蜘蛛香中缬草素的最佳工艺条件为：药材粉碎，过40目筛，浸泡30min后，用相当于药材8倍重量的95％乙醇提取3次，每次提取40min。结论：超声波提取法可用于蜘蛛香中缬草素的提取。
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| 关 键 词： | 蜘蛛香 缬草素 超声波提取 HPLC |
The Orthogonal Test for Optimization of the Valtrate Extracting Procedure from Valeriana Wallichii DC |
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| Chen Yujuan,Shi Jinli,Yah Xingli,Liu Yong,Xiao Peigen. The Orthogonal Test for Optimization of the Valtrate Extracting Procedure from Valeriana Wallichii DC[J]. World Science and Technology(Modernization of Traditional Chinese Medicine), 2009, 11(6): 889-891 |
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| Authors: | Chen Yujuan Shi Jinli Yah Xingli Liu Yong Xiao Peigen |
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| Affiliation: | (School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China) |
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| Abstract: | This work aimed to optimize the extracting process of valtrate from Valeriana wallichii DC （V. jatamansi Jones） by supersonic wave. The orthogonal experiment L9（3^4） was adopted, and the gaining rate of vahrate was used as the evaluating criterion. The optimal conditions were as follows： the plant material was powdered, passed through a 40 mesh sieve, soaked for 30min, and extracted for 3 times with 8-fold weight 95% ethanol for 40min each time. The results showed that supersonic wave is suitable for the extraction of valtrate from V. wallichii. |
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| Keywords: | HPLC |
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