氯化三丁基锡对小鼠肢芽细胞遗传毒性的实验研究

目的了解环境污染物氯化三丁基锡(TBTCl)对小鼠胚胎肢芽细胞的遗传毒性。方法将孕12 d昆明种小鼠颈椎脱臼处死,分离胚胎,切取肢牙,剪碎,0.125%胰酶消化,用无血清的Ham’s F12基础培养基将其制成单细胞悬液。加入不同浓度的TBTCl,使其终浓度分别为:0、0.1、0.2、0.4、0.8 mg/L。37.5℃恒温孵育2 h,取细胞悬液做彗星实验。结果TBTCl染毒组随染毒剂量的浓度的增高,肢芽细胞彗星拖尾率和尾长分别从对照组的4.0%和(9.7±4.3)μm上升到0.8 mg/L TBTCl组的61.7%和(26.9±12.5)μm,呈明显的剂量-反应(效应)关系。结论TBTCl能损伤昆明种小鼠胚胎肢芽细胞DNA,具有遗传毒性。

Developmental Genetic Toxicity Effect of Tributyltin Chloride on Mouse Embryonic Limb Bud Cells

Objective To investigate the genetic toxicity effect of tributyltin chloride （TBTCL） on mouse embryonic limb bud cells. Methods The embryos were removed from 12 - day stage pregnant mice, and limb bud cells of embryos were treated with TBTCL （the final concentration： 0.0 mg/L, 0.1 mg/L, 0.2 mg/L, 0.4 mg/L, and 0.8 mg/L） for 2 hours at 37.5 ℃. Then comet assay was used to investigate the damage to the DNA of limb bud cells. Results The rate of comet ceils and the tail length of comet cells increased with the increasing of TBTCl concentration, and there was apparent dosereaction relationship. Conclusion TBTOl could damage the DNA of mouse embryonic limb bud cells and has genetic toxicity to them.