天冬氨酸修饰纳米羟基磷灰石/聚乳酸复合物制备软骨组织工程支架及其生物性能研究

目的:通过天冬氨酸修饰的纳米羟基磷灰石（Asp-nHA）和左旋聚乳酸（PLLA）复合，制备软骨组织工程支架，并进行体外实验研究，探索其作为软骨组织工程支架的可行性。 方法:以碳二亚胺盐酸盐/N-羟基琥珀酰亚胺偶联法制备Asp-nHA，并与PLLA复合，获得新型Asp-nHA/PLLA纳米复合支架。比较成骨细胞在该新型材料表面的粘附、生长和增殖的情况。 结果:加入nHA后，Asp-nHA/PLLA复合材料可显著增强细胞的粘附、生长、增殖；Asp-nHA/PLLA的生物学性能明显优于PLLA及HA/PLLA，且Asp-nHA/PLLA具有最强的体外诱导成骨细胞分化能力。 结论:Asp-nHA/PLLA制备简单，具有良好的生物相容性和成骨活性，是一种性能良好的骨组织工程支架材料。

Preparation of bone tissue engineering scaffold with aspartic acid-modified nano-hydroxyapatite/poly L-lactic acid and its bio-characterizations

Abstract: Objective To design and prepare surface modified cartilage tissue scaffolds with the use of aspartic acid-modified nano-hydroxyapatite (Asp-nHA) and poly L-lactic acid (PLLA), and evaluate its feasibility as a scaffold for cartilage tissue engineering through in vitro experiment. Methods Asp-nHA was synthesized with the standard EDC/NHS coupling method, and the resultant Asp-nHA was conjugated to PLLA to form Asp-nHA/PLLA composite scaffold. The osteoblast adhesion, expansion and proliferation on Asp-nHA/PLLA composite scaffold were evaluated. Results Osteoblast adhesion, expansion and proliferation on Asp-nHA/PLLA composite scaffold were significantly increased due to the addition of nHA. The in vitro analysis illustrated that Asp-nHA/PLLA composite was superior to PLLA and HA/PLLA in bio-characterizations and had the strongest ability to induce osteogenic differentiation in vitro. Conclusion Asp-nHA/PLLA is simple to be prepared and its good biocompatibility and osteogenic activity make it become a good kind of scaffold for cartilage tissue engineering.