| 大鼠脑组织中儿茶酚胺类神经递质的rP—HPLC—ECD定量分析 |
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| 引用本文： | 张纬 祁艳斌. 大鼠脑组织中儿茶酚胺类神经递质的rP—HPLC—ECD定量分析[J]. 天津医科大学学报, 1998, 4(3): 218-219 |
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| 作者姓名： | 张纬 祁艳斌 |
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| 作者单位： | 天津医科大学生化教研室 |
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| 摘 要： | 目的：建立一种定量分析脑组织中儿茶酚胺类神经递质的方法。方法：采用反相高效液相色谱电化学色谱法（ｒＰ－ＨＰＬＣ－ＥＣＤ）测定大鼠脑组织中去甲肾上腺素（ＮＥ）、肾上腺素（Ｅ）、多巴胺（ＤＡ）、５羟色胺（５－ＨＴ）。结果：由色谱图显示各单胺类成分分离完全。结论：本方法快速、简便、准确，为医务工作者提供方便与帮助
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| 关 键 词： | 儿茶酚胺；HPLC；神经递质 |
THE QUANTITATIVE ANALYSIS OF CATECHOLAMINE NEUROTRANSMITTER IN RAT BRAIN BY Rp HPLC |
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| Zhang Wei,Qi Yanbin,Liu Demin. THE QUANTITATIVE ANALYSIS OF CATECHOLAMINE NEUROTRANSMITTER IN RAT BRAIN BY Rp HPLC[J]. Journal of Tianjin Medical University, 1998, 4(3): 218-219 |
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| Authors: | Zhang Wei Qi Yanbin Liu Demin |
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| Affiliation: | Tianjin Medical University 300070 |
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| Abstract: | Objective:To establish a method that can quantitatively determine catecholamine neurotransmitter in rat brain Methods:Reversed phase high performance liquid chromatorgraphy rP HPLC ECD) is used here to determine norepinephrine, epinephrine, dopamine, 5 hydroxytrptamine in rat brain Results:The chromatogram manifests that each kind of monoamine is completely separated Conclusion:This method is a quick, simple and reliable method It will provide great convenience to the people engaging in medical affairs |
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| Keywords: | Catecholamine HPLC Neurotransmitter |
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