大肠癌患者术中腹腔冲洗液中Survivin mRNA的检测及临床意义

目的 探讨检测大肠癌患者术中腹腔冲洗液Survivin mRNA表达的临床意义.方法 收集36例大肠癌和8例大肠良性病变患者的术中腹腔冲洗液,用RT-PCR方法 测定腹腔冲洗液中游离细胞的Survivin mRNA表达,同时作腹腔冲洗液细胞学检查(PLC).结果 腹腔冲洗液用RT-PCR方法 测定Survivin mRNA的阳性率为52.7%(19/36),高于腹腔冲洗液PLC的30.5%(11/36).Survivin mRNA的阳性率与肿瘤的组织学类型、淋巴结转移、浆膜侵犯程度、肿瘤分化程度和Dukes分期有关.结论 检测腹腔冲洗液中Survivin mRNA的表达将有助于大肠癌的预后的判断及治疗方案的制定.该法较PLC方法 有更高的灵敏度和特异性.

Detecting survivin mRNA in peritoneal lavage fluid of colorectal cancer patients and its clinical significance

Objective To explore the significance of using cytologic and RT-PCR methods to examine peritoneal washings of colorectal cancer patients in prediction of peritoneal micrometastasis.Methods The peritoneal washings of 36 patients with colorectal cancer and 8 patients with benign colorectal lesions were collected,Survivin mRNA expression of free cells in peritoneal washings were detected by RT-PCR method and also cytology of the washings were performed.Results The Survivin mRNA expression rate of peritoneal washings was 52.7%(19/36),which was more sensitive than that of cytologic examination 30.5%(11/36).Dukes staging,depth of invasion,lymph node metastasis,and serosal involvement were related to the expression rate of Survivin mRNA.Conclusions The detection of Survivin mRNA in peripheral blood is helpful to early diagnose,assess the prognosis and make a correct treatment of colorectal carcinoma.This method is more sensitive and specific than cytologic examination.