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| 缺血再灌注损伤诱导心肌细胞自噬相关基因过表达 | |
| 引用本文： | 肖健,何斌,朱晓燕,张宇峰,康波,王志农. 缺血再灌注损伤诱导心肌细胞自噬相关基因过表达[J]. 第二军医大学学报, 2011, 32(7): 776-779. DOI: 10.3724/SP.J.1008.2011.00776 |
| 作者姓名： | 肖健 何斌 朱晓燕 张宇峰 康波 王志农 |
| 作者单位： | 1. 第二军医大学长征医院胸心外科,上海,200003 2. 上海交通大学附属新华医院麻醉科,上海,200092 3. 第二军医大学基础部生理学教研室,上海,200433 |
| 基金项目： | 国家自然科学基金青年科学基金项目 |
| 摘 要： | 目的探讨缺血再灌注(IR)损伤对心肌细胞自噬相关基因(autophagy-related gene,Atg)表达的影响。方法成年雄性SD大鼠随机分为2组:对照组(假手术组),于左前降支(left anterior descending,LAD)下方穿过5-0丝线,不结扎;缺血再灌注损伤组:结扎LAD,45 min后松结扎线再灌注120 min。利用RT-PCR方法,检测各组心肌Beclin 1和Atg5mRNA表达;应用蛋白质印迹分析法检测心肌LC3蛋白表达变化。结果缺血再灌注损伤后心肌Beclin 1和Atg5 mRNA表达较对照组升高,分别达到对照组的2.45和1.6倍,差异具有统计学意义(P<0.05);LC3-Ⅱ蛋白表达增加,IR后LC3-Ⅱ/LC3-Ⅰ比值较对照组升高(1.4 vs 0.2,P<0.01)。结论缺血再灌注损伤可以诱导心肌细胞Atg家族表达增加,促进自噬的发生,导致心肌损害。 |
| 关 键 词： | 心肌再灌注损伤 心肌细胞 自噬 |
| 收稿时间： | 2010-12-07 |
| 修稿时间： | 2011-06-22 |
Ischemia-reperfusion injury induced overexpression of autophagy-related genes in cardiomyocytes |
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| XIAO Jian,HE Bin,ZHU Xiao-yan,ZHANG Yu-feng,KANG Bo and WANG Zhi-nong. Ischemia-reperfusion injury induced overexpression of autophagy-related genes in cardiomyocytes[J]. Former Academic Journal of Second Military Medical University, 2011, 32(7): 776-779. DOI: 10.3724/SP.J.1008.2011.00776 | |
| Authors: | XIAO Jian HE Bin ZHU Xiao-yan ZHANG Yu-feng KANG Bo WANG Zhi-nong |
| Affiliation: | 1. Department of Cardiothoracic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;3. Department of Anaesthesiology, Xinhua Hospital, Shanghai Jiaotong University, Shanghai 200092, China;2. Department of Physiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China |
| Abstract: | Objective To investigate the effect of ischemia-reperfusion(IR) injury on the expression of autophagy-related gene in cardiomyocytes.Methods Male adult SD rats,weighing 250-300 g,were randomly divided into 2 groups,namely,the control group,in which rats underwent thoracotomy without left anterior descending coronary(LAD) ligation,and IR group,in which the left anterior descending coronary arteries were ligated for 45 min,followed by reperfusion for 2 h.Beclin 1 and Atg5 mRNA expression levels were examined ... |
| Keywords: | myocardial reperfusion injury myocardiocytes autophagy |
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