日本血吸虫成虫培养细胞骨架系统的研究

目的：研究日本血吸虫成虫培养细胞的骨架系统。方法：将日本血吸虫成虫细胞接种于小盖玻片上，以RPMI－1640含20％小牛血清附加常量抗菌素的培养基培养，用考马斯亮蓝染色法和魁笔环肽荧光染色法显示培养细胞骨架。结果：考马斯亮蓝染色显示日本血吸虫成虫细胞骨架呈网络状结构，均钭分布于胞质中；用（NH4）2SO4抽提后考马斯亮蓝色显示细胞骨架无明显改变；鬼笔环肽荧光染色后可见细胞内有均匀荧光亮点。结论：日本血吸虫成虫培养细胞骨架呈致密网络状结构，以中间纤维为主，缺乏粗大的微丝束。

STUDIES ON CYTOSKELETON OF CULTURED CELLS FROM ADULT SCHISTOSOMA JAPONICUM

Objectives To study cytoskeleton of cultured cells from adult Schistosoma japonicum .Methods The cells were inoculated on small glass coverslips and cultured with RPMI-1640 contained 20% calf serum.The coverslips with the cells were moved out for being used for observing cytoskeleton by Coomassie blue staining and Rhodamin-Phalloidin fluorescence staining.Results By Coomassie blue staining,cytoskeleton of cultured cells was revealed that it was correspondingly uniform and tight network-like structure in the cytoplasm ,so was cytoskeleton of cultured cells treated by (NH 4) 2SO 4. There were even fluorescence dots in the cells by Rhodamin-Phalloidin fluorescence staining. Conclusion Cytoskeleton of cultured cells from adult S. japonicum appeared tight network-like structure,absent of thick microfilament bundles and was prinicipally constituted by intermediate filaments.