成年糖尿病大鼠睾丸、附睾和前列腺内雄激素受体的表达研究

目的:观察糖尿病大鼠睾丸、附睾和前列腺内是否存在雄激素受体(AR)的异常表达。方法:用链脲菌素(STZ)诱导成年大鼠糖尿病模型,实验分正常对照组(C组)、糖尿病组(D组)及胰岛素治疗组(ID组),分别以Northern印迹及放射配基法检测睾丸、附睾和前列腺内AR的mRNA及蛋白质水平。结果:D和ID组血清睾酮水平低于C组(P<0.05);附睾内D和ID组AR的mRNA水平低于C组(P<0.05),睾丸和前列腺内D组AR的蛋白质水平低于C组(P<0.05)。结论:糖尿病大鼠睾丸、附睾和前列腺内AR的表达降低,从而削弱了雄激素的生物利用度,这可能是导致患病大鼠性与生殖功能障碍的原因之一。

Study on the Expression of Androgen Receptor in Testis, Epididymis and Prostate of Adult Rats with Diabetes

Objective: To investigate if there is abnormal expression of androgen receptor(AR) in adult diabetic rats.Methods: The diabetic model rats were induced by using streptozotocin(STZ),which were divided into three groups: control(group C),diabetes(group D) and diabetes with insulin replacement group(group ID).The mRNA and protein expressions of androgen receptor in testis,epididymis and prostate were detected by Northern blot and radioimmunoassay,respectively.Results: Serum testosterone levels and AR mRNA in epididymis of group D or Group ID were lower than those of group C(P<(0.05)),respectively,and the protein expression of AR in testis and prostate of group D was lower than that of group C(P<(0.05)).Conclusion: The expression of androgen receptor decreased in testis,epididymis and prostate of diabetic rats,which weakened the biological effects of AR,and it might be one of the causes that resulted in the sexual and productive dysfunction in diabetic rats.