| 改良型TAT-VP3融合蛋白的表达、纯化及鉴定 |
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| 引用本文： | 张启波,王剑松,詹辉,颜汝平,杨峻峰,李俊.改良型TAT-VP3融合蛋白的表达、纯化及鉴定[J].昆明医学院学报,2010,31(9):4-6,26. |
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| 作者姓名： | 张启波 王剑松 詹辉 颜汝平 杨峻峰 李俊 |
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| 作者单位： | 1. 昆明医学院第二附属医院泌尿外科,云南,昆明,650101
2. 昆明医学院第一附属医院肾内科,云南,昆明,650032 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的表达改良型TAT-VP3融合蛋白,并对其进行纯化.方法利用IPTG温控诱导改良型TAT-VP3融合蛋白表达,并经GST亲和层析纯化,目的蛋白进行SDS-PAGE和Western bolt鉴定.结果改良型TAT-VP3融合蛋白经温控诱导后得到稳定表达,经SDS-PAGE鉴定可见相对分子质量42 kD的特异性目的条带,经GST亲和层析纯化后,得到了纯度较高的目的蛋白.Western bolt验证了表达蛋白的特异性.结论已成功表达及纯化出改良型TAT-VP3融合蛋白,为进一步研究其免疫原性和抗肿瘤活性奠定了基础.
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| 关 键 词： | 改良型TAT-VP3融合蛋白 表达 纯化 鉴定 |
Expression ,Purification and Identification of Improved TAT-VP3 Fusion Protein |
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| ZHANG Qi-bo,WANG Jian-song,ZHAN Hui,YAN Ru-ping,YANG Jun-feng,LI Jun.Expression ,Purification and Identification of Improved TAT-VP3 Fusion Protein[J].Journal of Kunming Medical College,2010,31(9):4-6,26. |
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| Authors: | ZHANG Qi-bo WANG Jian-song ZHAN Hui YAN Ru-ping YANG Jun-feng LI Jun |
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| Institution: | ZHANG Qi-bo1),WANG Jian-song1),ZHAN Hui1),YAN Ru-ping1),YANG Jun-feng1),LI Jun2)(1)Dept.of Urology,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,2)Dept.of Nephrology,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China) |
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| Abstract: | Objective To express and purify improved TAT-VP3 fusion protein.Methods The improved TAT-VP3 fusion protein was expressed under the induction of temperature by IPTG.The expressed product was purified by GST affinity chromatography and identified by SDS-PAGE and Western blot.Results The improved TAT-VP3 fusion protein was stably expressed under the induction of temperature.The specific band with relative molecular masses of 42000 was identified by SDS-PAGE.The expressed product was purified successfully by G... |
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| Keywords: | Improved TAT-VP3 fusion protein Expression Purification Identification |
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