白背叶根抗鸭乙型肝炎病毒的实验研究

目的:观察白背叶根体内抗鸭乙型肝炎病毒(duck hepatitis B virus,D-HBV)的作用。方法:采用PCR技术筛选D-HBV阳性3日龄广州麻鸭40只。将麻鸭随机分为空白对照组,白背叶根高、中、低剂量组和拉米夫定组,分别给予相应药物进行干预。用药前、用药第7、14、21天及停药第7天,取各组麻鸭静脉血,采用实时定量荧光PCR技术检测血清D-HBV DNA含量;治疗前后取肝脏组织进行病理组织学观察。结果:拉米夫定组于用药后,血清病毒水平迅速降低,但停药后存在反跳现象。白背叶根高、中剂量组分别于治疗第14、21天其血清病毒含量明显下降,低剂量组治疗期间未见明显的抑制病毒作用。停药后,白背叶根高、中剂量组与空白对照组比较仍表现出一定的病毒抑制作用。白背叶根高剂量组对肝脏炎症的改善作用较拉米夫定组明显。结论:白背叶根有抑制体内D-HBV复制的作用,其作用大小与剂量及用药时间相关;其治疗作用较拉米夫定弱,但作用维持时间长,且用药较为安全。

Inhibiting effects of root of Mallotus apelta on duck hepatitis B virus

Objective: To evaluate the inhibiting effects of the root of Mallotus apelta(Lour.) Muell.-Arg.on duck hepatitis B virus(D-HBV) in vivo.Methods: Forty nestling ducks with congenitally infection of D-HBV detected by PCR were randomly divided into five groups: untreated group,lamivudine-treated group,and high-,medium-and low-dose root of Mallotus apelta-treated groups.The ducks in the lamivudine-treated group were fed lamivudine with a dose of 50 mg/kg once.Ducks in the three-dose Mallotus apeltatreated groups were treated with different doses of decoction of this herbal medicine for 21 days respectively.The serum content of D-HBV DNA was determined by quantitative real-time PCR technique before and 7 days after the treatment, and on the 7th,14th and 21st day of the treatment.Liver(biopsy) was also executed(before) and after the treatment to observe the histopathological changes.Results: Lamivudine showed a rapid inhibiting effect on D-HBV DNA,but this effect didn't last long,and the serum level of D-HBV DNA increased again(after) treatment.The serum level of D-HBV DNA dropped markedly in the high-and medium-dose Mallotus apelta-treated groups on the 14th and 21st day.Low-dose Mallotus apelta revealed no obvious inhibiting effect on D-HBV.After treatment,the inhibiting effect in the root of Mallotus apelta-treated group continued as compared with that in the untreated group.The histopathological changes of liver tissues showed that the inflammation in the high-dose root of Mallotus apelta-treated group was weakened as compared with that in the lamivudine-treated group.Conclusion: The root of Mallotus apelta has therapeutic effect on D-HBV.It can restrain the duplication of D-HBV in vivo.Although this effect is weaker than that of lamivudine,it continues longer.Thus this herbal medicine is an effective,safe and economical drug for hepatitis B.