实时荧光定量PCR检测血浆结核分枝杆菌DNA的初步临床应用

目的 评价实时荧光定量PCR技术检测血浆（或血清）结核分枝杆菌（Mycobacterium tuberculosis，MTB）DNA的技术。方法 建立实时荧光定期量PCR方法测定血浆MTB DNA，分别检测临床确诊的55例结核病患者血清43份，血浆25份以及非结核肺部疾病患者血清18份，健康体检血清18份和健康体检者血浆11份的MTB DNA含量。结果 18例非结板肺疾病患者血清、18例健康体检者血清以及11例健康体检者血浆MTB DNA全部阴性。55例初诊结核患者中10例（18．2％）治疗前血浆（或血清）MTB DNA阳性。在痰涂片阴性的18例结核患者中，血浆（或血清）MTB DNA阳性检出率为27．8％（5／18），其特异性达100％。结论 本研究证实了结核患者血浆（或血清）循环MTB DNA的存在，其定量检测对痰涂片阴性及无痰患者的结核病诊断有重要参考价值。

Clinical application of quantification of Mycobacterium tuberculosis DNA in plasma by real-time PCR

Objective To evaluate the value of quantitative fluorescent PCR for detection of Mycobacterium tuberculosis(MTB)DNA in plasma(or serum).Methods The content of MTB DNA in the following samples was detected by real-time PCR,including 43 sera and 25 plasmas from tuberculosis patients,18 sera from the patients with non-tuberculosis pulmonary disease and 18 sera and 11 plasmas from healthy volunteers.Results MTB DNA was negative in 18 sera from the patients with non-tuberculosis pulmonary disease and all the specimens(serum and plasma)from healthy volunteers.The positive rate of MTB DNA in 55 tuberculosis patients was 18.2%(10/55).The positive rate was 27.8% in smear-negative TB patients compared with 13.5% in smear-positive TB patient group.The specificity of the detection of MTB DNA was 100%.Conclusions This study confirmed the existence of circulating MTB DNA in plasma and serum of tuberculosis patients and showed that the detection of MTB DNA may provide valuable information for the diagnosis of smear-negative TB patients.