| HPLC法测定肿节风中异秦皮啶含量 |
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| 引用本文： | 张萍,李军,马双成,王钢力,林瑞超.HPLC法测定肿节风中异秦皮啶含量[J].中国药事,2009,23(6):538-540. |
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| 作者姓名： | 张萍 李军 马双成 王钢力 林瑞超 |
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| 作者单位： | 1. 中国药品生物制品检定所,北京,100050
2. 安徽省亳州药品检验所 |
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| 摘 要： | 目的测定肿节风药材中异秦皮啶含量。方法样品经甲醇加热回流提取,采用HPLC法测定。色谱柱为Alltima C18柱;流动相：乙腈-0.1%磷酸溶液（20∶80）;流速：1.0mL.min-1;检测波长：344nm。结果异秦皮啶在0.021~0.128μg范围内具有良好的线性关系（r=0.9994）;平均加样回收率（n=5）为99.4%,RSD=2.62%。结论本方法操作简便、快捷,灵敏度高,可用于肿节风中异秦皮啶的含量测定。
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| 关 键 词： | 肿节风 异秦皮啶 HPLC |
Determination of Isofraxidin in Sarcandra glabra (Thunb.)Nakai by HPLC |
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| Zhang Ping,Li Jun,Ma Shuangcheng,Wang Gangli,Lin Ruichao.Determination of Isofraxidin in Sarcandra glabra (Thunb.)Nakai by HPLC[J].Chinese Pharmaceutical Affairs,2009,23(6):538-540. |
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| Authors: | Zhang Ping Li Jun Ma Shuangcheng Wang Gangli Lin Ruichao |
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| Institution: | Zhang Ping, Li Jun, Ma Shuangcheng, Wang Gangli and Lin Ruichao(1 National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050; 2 Anhui Province Bozhou Institute for Drugs Control) |
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| Abstract: | Objective To determine isofraxidin in Sarcandra glabra （Thunb.） Nakai. Methods The contents of isofraxidin in Sarcandra glabra （Thunb.） Nakai were assayed by HPLC. The column used was Alltima C18 column, the mobile phase was acetonitrile-0. 1% phosphoric acid （20 ： 80） at a flow rate of 1.0mL · min^-1, the detection wavelength was set at 344nm. Results The linear range was 0. 021 0. 128μg （r=0. 9994）. The average recovery （n= 5） of isofraxidin was 99.4%, RSD was 2.62%. Conclusions The method is effective and accurate. It can be applied to determination of isofraxidin in Sarcandra glabra （Thunb.） Nakai. |
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| Keywords: | HPLC |
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