甘肃省河西走廊地区2011年蚊虫种类及虫媒病毒调查研究

目的调查甘肃省河西走廊地区蚊虫种类及所携带虫媒病毒分布状况。方法使用诱蚊灯于18:00至次日06:00进行蚊虫标本采集,分类鉴定后液氮保存并运送至实验室备检;通过组织培养法分离病毒,并对病毒分离物进行血清学和分子生物学鉴定;利用生物信息学技术对新分离病毒的序列进行分析,完成同源性和系统发生分析。结果 2011年8月在甘肃省河西走廊地区7个县(市)共采集到蚊虫标本3属6种24028只,其中刺扰伊蚊最多,占采集总数的32.80%(7880/24028),淡色库蚊占30.26%(7272/24028);通过细胞培养分离到1株可以引起两种细胞(BHK-21和C6/36细胞)病变的病毒分离株(GS11-155);此外在32批蚊虫标本中检测到病毒(库蚊黄病毒、盖塔病毒、辽宁病毒)基因阳性,对其中11批核苷酸序列测定和分析发现,1批为蚊传黄病毒,3批为盖塔病毒,7批为辽宁病毒。结论甘肃省河西走廊地区优势蚊种为刺扰伊蚊,且蚊虫携带多种虫媒病毒,包括蚊传黄病毒、盖塔病毒和辽宁病毒。

Investigation of mosquitoes and arboviruses in Hexi Corridor of Gansu province,China in 2011

Objective To investigate the species of mosquitoes and the distribution of arboviruses carried by the mosquitoes in Hexi Corridor of Gansu province,China in 2011.Methods Mosquito samples were collected from 6 p.m.to 6 a.m.in the next day with UV lamps.After being identified,the samples were preserved in liquid nitrogen and then transported to a laboratory for examination.Viruses were isolated from the samples by tissue culture,and the virus isolates were identified by serological and molecular biological methods.Homology and phylogenetic analysis was performed on the nucleotide sequences of isolated viruses by bioinformatics.Results A total of 24 028 mosquitoes,belonging to 6 species and 3 genera,were collected in seven counties or cities along Hexi Corridor in August 2011.Among the collected mosquitoes,Aedes vexans was the most abundant species,accounting for 32.80%(7880/24 028),and Culex pipiens pallens accounted for 30.26%(7272/24 028).One virus strain(GS11-155) that could induce pathological changes of two types of cells(BHK-21 and C6/36 cells) was isolated by cell culture.Genes of Culex flavivirus(CxFV),Liaoning virus(LNV),and Getah virus(GETV) were detected in 32 batches of mosquito samples,and in 11 of the 32 batches,1 had CxFV,3 had GETV,and 7 had LNV,according to nucleotide sequencing.Conclusion Ae.vexans was the dominant species of mosquitoes in Hexi Corridor of Gansu province,China.The mosquitoes in Hexi Corridor carry several types of arboviruses,including the CxFV,GETV,and LNV.